不同培养液对大菱鲆致病性纤毛虫——贪食迈阿密虫种群增长及复壮毒力的影响

从山东烟台地区某大菱鲆养殖场的患病大菱鲆体内分离出致病性纤毛虫——贪食迈阿密虫,筛选了4种盾纤毛虫常用体外培养液[大肠杆菌培养液(DH)、牛肉浸膏培养液(BEV)、米粒培养液(RV)、改良L-15培养液(L-15)],研究了不同培养基对纤毛虫种群增长及复壮毒力的影响.试验结果显示:在BEV和L-15两种培养液中,贪食迈...

Effects of different culture media on population growth and virulence rejuvenation of pathogenetic ciliate (Miamiensis avidus) in turbot(Scophthalmus maximus)

In recent years, studies on the isolation and culture of marine ciliates were limited to the effects of different culture media on population growth,and there were few reports on the toxicological data of pathogenetic ciliates in different culture media.In this experiment,the effects of four culture medias on population growth and virulence rejuvenation of ciliate(Miamiensis avidus) isolated from turbot(Scophthalmus maximus) were studied.The results showed that the density peak of population density was observed earliest in both beef extract culture medium and the modified L-15 group.The density peak in E. coli culture medium and rice grain culture medium was observed 24 hours later.The modified L-15 group had the highest population density with longer stable period and later decay period,and the rice culture medium came next.The virulence rejuvenation test of M. avidus showed that the modified L-15 culture fluid was the most suitable parasitic environment for M. avidus,and the beef extract culture was the second.