A Brief Overview of Transgenic Farm Animals |
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Authors: | Bacci M. L. |
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Affiliation: | (1) Department of Animal Biotechnology, Madras Veterinary College, Vepery, Chennai, 600 007;(2) National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, India |
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Abstract: | A rapid recombinant antigen-based latex agglutination test (LAT) has been developed to detect specific anti-leptospiral antibodies from human and dog sera. The recombinant LipL32 antigen developed and used for detecting the antibodies is specific in detection of the pathogenic serovars of Leptospira as the expression of the LipL32 antigen is restricted only to the pathogenic leptospires. The sensitized latex beads are stable and could be stored at 4°C for more than three months without showing loss of activity for both weakly and strongly positive samples. The test is found to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT). Moreover, the recombinant antigen-coated latex beads could detect the specific anti-leptospiral antibodies in the acute phase of the illness. The test is simple and inexpensive, and is rapid in the management of large numbers of patients. |
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Keywords: | leptospirosis pathogenic recombinant LipL32 antigen latex agglutination test diagnosis |
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