利用糊粉层激素敏感性筛选小麦α-淀粉酶变异体 |
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引用本文: | 黄穗,郑企成.利用糊粉层激素敏感性筛选小麦α-淀粉酶变异体[J].核农学报,1988,2(1):13-18. |
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作者姓名: | 黄穗 郑企成 |
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作者单位: | 中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所 北京
,北京 |
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摘 要: | 以小麦种子为试材,研究筛选种子胚乳糊粉层中α-淀粉酶调控机制变异体的方法。应用这种方法,在花培纯系内籽粒间未发现变异;在体细胞无性系的籽粒间发现了对激素敏感性改变的变异体。表明了该筛选方法的可行性。 本文还对适宜的试验条件和筛选指标作了探讨。
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关 键 词: | 胚乳 α-淀粉酶 生化变异体 |
收稿时间: | 2009-12-31 |
修稿时间: | 2009-12-31 |
SCREENING FOR WHEAT a-AMYLASE VARIANT WITH ALTERED HORMONE SENSITIVITY IN THEIR ALEURONE LAYERS |
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Abstract: | A method for screening the variant of α-amylase regulation mechanism in the aleurone layers of wheat seeds was developed.Using this method, no variation of the abilitv on synthesizing and secreting a-amylase was observed among grains of homozygous wheat plants (H2)derived from anther culture; but some variations with altered hormone sensitivity occurred among somaclonal plants(SC1).The result showed that this screening method is feasible.Factors appropriate for the experiment and selection were also investigated |
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Keywords: | endosperm a-amylase biochemical variation |
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