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| 根癌农杆菌介导的桃幼胚转化实验参数研究 | |
| 引用本文： | 吴延军,张上隆,徐昌杰,李南羿,金勇丰. 根癌农杆菌介导的桃幼胚转化实验参数研究[J]. 果树学报, 2004, 21(5): 477-479 |
| 作者姓名： | 吴延军 张上隆 徐昌杰 李南羿 金勇丰 |
| 作者单位： | 1. 浙江大学园艺系,浙江杭州,310029;安徽农业大学园艺系,安徽合肥,230036 2. 浙江大学园艺系,浙江杭州,310029 3. 浙江大学生物技术研究所,浙江杭州,310029 |
| 基金项目： | 宁波市科委项目支持桃转基因研究(2000农业项目资助)，安徽省教委(2002kj086)项目 |
| 摘 要： | 以桃幼胚作为根癌农杆菌介导转化的受体,通过对GUS基因瞬时表达率的分析,研究此转化体系的最佳实验参数。实验结果表明,预培养时间、感染时间、共培养时间和根癌农杆菌诱导物AS等对转化效率都有一定的影响。预培养1d、感染15min、共培养42h和不添加AS时GUS瞬时表达率最高。 |
| 关 键 词： | 桃 幼胚 转化 GUS |
| 文章编号： | 1009-9980(2004)05-477-03 |
| 修稿时间： | 2004-02-01 |
Studies on the Optimal Conditions for Peach (Prunus persica) Immature Embryos Transformed by Agrobacterium tumefaciens |
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| Wu Yanjun,,Zhang Shanglong,Xu Changjie,Li Nanyi,and Jin Yongfeng. Studies on the Optimal Conditions for Peach (Prunus persica) Immature Embryos Transformed by Agrobacterium tumefaciens[J]. Journal of Fruit Science, 2004, 21(5): 477-479 | |
| Authors: | Wu Yanjun Zhang Shanglong Xu Changjie Li Nanyi and Jin Yongfeng |
| Affiliation: | Wu Yanjun1,2,Zhang Shanglong1*,Xu Changjie1,Li Nanyi3,and Jin Yongfeng1 |
| Abstract: | Using transient expression of GUS gene,the optimal conditions for Agrobacterium-mediated transformation of peach immature embryos was investigated. Histochemical GUS assay showed that the transient expression rate was infected by pre-culture time, infection time, co-culture time and the way of adding acetosyringone. Pre-culture 1 d, infection 10 to 15 min, co-culture 42 h and no add of acetosyringone contributed to the highest level of transient expression. |
| Keywords: | Peach Immature embryos Transformation GUS |
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