培养条件对甘草不同外植体愈伤组织中黄酮形成的影响 |
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引用本文: | 曹君迈,赵海燕,张嫱,王晓蕾.培养条件对甘草不同外植体愈伤组织中黄酮形成的影响[J].安徽农业科学,2009,37(3):985-987. |
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作者姓名: | 曹君迈 赵海燕 张嫱 王晓蕾 |
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作者单位: | 北方民族大学生命科学与工程学院,宁夏银川,750021;北方民族大学生命科学与工程学院,宁夏银川,750021;北方民族大学生命科学与工程学院,宁夏银川,750021;北方民族大学生命科学与工程学院,宁夏银川,750021 |
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摘 要: | 目的]筛选生产次生代谢产物黄酮的最佳外植体和培养基配方及培养条件。方法]采用L1(4^5)正交试验设计和对比试验设计方法,研究ZT、ABA、Kr、6-BA和NAA5种外源激素在不同培养条件下对由甘草根和芽诱导的愈伤组织中黄酮形成的影响。结果]测验表明,弱光照条件(100~300Ix)有利于黄酮产量的形成;根诱导的愈伤组织是黄酮产量形成的适宜外植体。F检验表明,5种外源激素对黄酮产量的形成存在极显著差异,对根诱导的愈伤组织中总黄酮含量的影响顺序是:KT〉6-BA〉ZT〉ABA〉NAA;对芽诱导的愈伤组织中总黄酮含量影响顺序是:6-BA〉KT〉NAA〉ABA〉ZT。经过优选获得最佳激素组合为:ABA 1.0mg/L+KT 2.0mg/L+ZT 1.0mg/L+6-BA 1.0mg/L+NAA 2.0mg/L.结论]该研究为工业化生产次生代谢产物黄酮提供了理论依据。
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关 键 词: | 甘草 外植体 培养条件 总黄酮 |
Effects of Cultural Conditions on Flavonoids Formation in Different Explant Callus of Glycyrrhiza uralensis Fisch |
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Institution: | CAO Jun-mai et al (College of Life Sciences and Engineering, North Nationality University, Yinchuan, Ningxia 750021) |
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Abstract: | Objective] The research aimed to screen the best explant,medium formula and cultural conditions for producing flavonoids of secondary metabolites.Method] Under different cultural conditions,the effect of 5 exogenous hormones of ZT,ABA,NAA,KT and 6-BA on formation of flavonoids in callus induced by roots and buds of Glycyrrhiza uralensis Fisch was studied by using the methods of orthogonal experiment design of L16(45) and contrast experiment design.Result] The T-test indicated that weak light(100-300 lx) was beneficial to forming of flavonoids production;the callus induced from root was a suitable explant for forming of flavonoids yield.F-test showed that 5 exogenous hormones had extremely significant difference on forming of flavonoids production,the effect on total flavonoids content in the callus induced by root was in the such order as: KT>6-BA>ZT>ABA>NAA;the effect on total flavonoids content in the callus induced by bud was in the such sequence as: 6-BA>KT>NAA>ABA>ZT.The optimal hormone combinations obtained after optimization were: ABA 1.0 mg/L + KT 2.0 mg/L +ZT 1.0 mg/L +6-BA1.0 mg/L + NAA 2.0 mg/L.Conclusion] The study provided a theoretical basis for industrialized production of secondary metabolites of flavonoids. |
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Keywords: | Glycyrrhiza uralensis Fisch Explant Cultural condition Total flavonoids |
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