| 饲用溶菌酶对吉富罗非鱼消化道组织结构和营养物质消化吸收的影响 |
| |
| 引用本文： | 王坛,赵金鑫,刘东来,孔纯,华雪铭,吴钊,王刚,冯悦,杨景丰,刘韬. 饲用溶菌酶对吉富罗非鱼消化道组织结构和营养物质消化吸收的影响[J]. 渔业科学进展, 2019, 40(6): 76-87 |
| |
| 作者姓名： | 王坛 赵金鑫 刘东来 孔纯 华雪铭 吴钊 王刚 冯悦 杨景丰 刘韬 |
| |
| 作者单位： | 上海海洋大学农业农村部鱼类营养与环境生态研究 中心上海201306;广东海大集团股份有限公司 广州 511400;广东海大集团股份有限公司 广州 511400;安徽省巢湖市中旱镇农业综合服务中心水产站 巢湖 238054;上海海洋大学农业农村部鱼类营养与环境生态研究 中心上海201306;上海海洋大学农业农村部鱼类营养与环境生态研究 中心上海201306;上海海洋大学农业农村部淡水水产种质资源重点实验室 上海201306;上海海洋大学水产科学国家级实验教学示范中心 上海201306 |
| |
| 基金项目： | 广东省教育厅产学研结合项目 |
| |
| 摘 要： | 为了解饲用溶菌酶对吉富罗非鱼(Oreochromis niloticus)消化道组织结构发育和营养物质利用的影响，本研究设计5种饲料溶菌酶添加水平：18 mg/kg (L18)、36 mg/kg (L36)、54 mg/kg (L54)、72 mg/kg (L72)和90 mg/kg (L90)，以不添加溶菌酶的饲料(L0)为对照，进行为期60 d的饲养。结果显示，饲料中添加溶菌酶对吉富罗非鱼肝脏和胃肠道消化酶活性产生了不同程度的影响。L36、L54和L72组的胃、前肠和中肠蛋白酶活性显著高于对照组(P<0.05)，肝脏、前肠脂肪酶活性在L54和L72组均显著高于对照组(P<0.05)，淀粉酶活性在除前肠、中肠外的L72和L90组显著高于对照组(P<0.05)。L36、L54和L72组鱼的肠道不同部位肠绒毛密度、高度和宽度较对照组显著提高，在肌层厚度上，溶菌酶添加组在前肠和后肠部位有下降趋势，L18、L36和L90组显著低于对照组(P<0.05)，而在中肠部位，肌层厚度随添加水平升高而呈先升后降的变化趋势，L36、L54和L72组在数值上高于对照组，但无显著性差异(P>0.05)；杯状细胞数量在L54和L72组均显著高于对照组(P<0.05)。肝脏切片图显示，L36和L54组肝细胞排列整齐、形状饱满，相较于对照组更致密，但高剂量添加组的肝脏健康程度有下降趋势。消化吸收率方面，在饲养Ⅰ期和Ⅱ期，L36和L54组对粗蛋白的表观消化率显著高于对照组，到饲养Ⅲ期和Ⅳ期，L36~L90组对干物质、粗蛋白和粗脂肪的消化吸收率均有显著提高(P<0.05)。研究表明，在本实验条件下，36、54 mg/kg溶菌酶添加水平具有最稳定的作用效果，吉富罗非鱼通过肝肠发育和消化酶活性的提高来促进对饲料干物质、粗蛋白和粗脂肪的消化吸收。
|
| 关 键 词： | 吉富罗非鱼 溶菌酶 消化吸收率 消化酶 肝肠组织形态 |
| 收稿时间： | 2018-09-18 |
| 修稿时间： | 2018-10-21 |
Effects of Dietary Lysozyme on the Digestive Tract Structure and Nutrient Digestibility of GIFT Tilapia (Oreochromis niloticus) |
| |
| WANG Tan,ZHAO Jinxin,LIU Donglai,KONG Chun,HUA Xueming,WU Zhao,WANG Gang,FENG Yue,YANG Jingfeng and LIU Tao. Effects of Dietary Lysozyme on the Digestive Tract Structure and Nutrient Digestibility of GIFT Tilapia (Oreochromis niloticus)[J]. Progress in Fishery Sciences, 2019, 40(6): 76-87 |
| |
| Authors: | WANG Tan ZHAO Jinxin LIU Donglai KONG Chun HUA Xueming WU Zhao WANG Gang FENG Yue YANG Jingfeng LIU Tao |
| |
| Affiliation: | Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; Guangdong Haid Group Co., Ltd, Guangzhou 511400,Guangdong Haid Group Co., Ltd, Guangzhou 511400,Zhonghan Town Fishery Station of Agricultural Comprehensive Service Center of Chaohu Anhui Province, Chaohu 238054,Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306,Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306,Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306,Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306,Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306,Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306 and Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306 |
| |
| Abstract: | |
| |
| Keywords: | GIFT tilapia Lysozyme Nutrient digestibility Digestive enzyme Digestive tract morphology |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《渔业科学进展》浏览原始摘要信息 |
|
点击此处可从《渔业科学进展》下载免费的PDF全文 |
|
