| 甘薯贮藏蛋白A基因5’端序列的克隆与分析(英文) |
| |
| 作者单位： | 南京师范大学生命科学学院 江苏南京210046(王登瞻,李荣鹏),淮阴师范学院生物系 江苏淮安223300(季勤,陈敏) |
| |
| 摘 要： | [Objective] The aim of the study is to clone the 5’ flanking region of Sporamin A gene from sweet potato. [Method] The sweet potato "Xushu 18" was used to amplify the 5’ flanking region of Sporamin A gene using specifically designed primers and then target fragment was analyzed by PLACE and PlantCare online. [Result] Besides the conserved elements including TATA-box and CAAT-box,the cis-acting elements including sucrose responsive element CMSRE1,SP8 acting site and some other regulatory sequence such as MYB binding site were also found in Spo A promoter sequence. The results suggest that the promoter has sucrose responsive function.[Conclusion] The study provided reference to reveal the regulation law of Spo A,and to develop high-level expression vectors promoted by Spo A promoter.
|
Cloning and Analysis of 5' Flanking Region of Sporamin A Gene from Sweet Potato |
| |
| Abstract: | [Objective] The aim of the study is to clone the 5’ flanking region of Sporamin A gene from sweet potato. [Method] The sweet potato "Xushu 18" was used to amplify the 5’ flanking region of Sporamin A gene using specifically designed primers and then target fragment was analyzed by PLACE and PlantCare online. [Result] Besides the conserved elements including TATA-box and CAAT-box,the cis-acting elements including sucrose responsive element CMSRE1,SP8 acting site and some other regulatory sequence such as MYB binding site were also found in Spo A promoter sequence. The results suggest that the promoter has sucrose responsive function.[Conclusion] The study provided reference to reveal the regulation law of Spo A,and to develop high-level expression vectors promoted by Spo A promoter. |
| |
| Keywords: | Sproramin A gene Sweet potato |
| 本文献已被 CNKI 等数据库收录！ |
|
