高致病性蓝耳病纯化灭活疫苗的研制(英文) |
| |
引用本文: | 唐艳林,;宋睿,;李静姬,;苗丽娟,;何玉友.高致病性蓝耳病纯化灭活疫苗的研制(英文)[J].农业科学与技术,2014(6):937-941. |
| |
作者姓名: | 唐艳林 ;宋睿 ;李静姬 ;苗丽娟 ;何玉友 |
| |
作者单位: | [1]吉林农业科技学院,吉林吉林132101; [2]蔚蓝生物股份有限公司,山东青岛266000; [3]吉林正业生物制品有限公司,吉林吉林132101 |
| |
基金项目: | 吉林市科技赶展计划(2013210029):吉林农业科技学院重点学科资助项目(2013x023), |
| |
摘 要: | 目的]改进和提高现行高致病性蓝耳病(HR-PRRS)灭活疫苗的纯度和效价。方法]该研究将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍,灭活后用Sepharose 4 Fast Flow分子筛柱层析,收集完整的病毒粒子,加油佐剂制成纯化HP-PRRS灭活疫苗。结果]通过纯化可将HP-PRRS病毒与非病毒蛋白分开,纯化后测定病毒蛋白含量收率为76.7%~82.4%,杂蛋白含量去除率均为96%以上,且纯化疫苗的攻毒保护率达到4/5以上,血清抗体检测阳转率达86%以上,均高于未纯化灭活疫苗,差异显著。结论]HR-PRRS病毒液经浓缩纯化制成疫苗后,增强了免疫效果,并可有效地去除杂蛋白,避免了疫苗的过敏反应和应激反应。
|
关 键 词: | 高致病性蓝耳病纯化灭活疫苗 浓缩纯化 攻毒保护率 抗体阳转率 应激反应 |
Preparation of Purified and Inactivated HP-PRRS Vaccine |
| |
Institution: | Yanlin TANG, Rui SONG, Jinji LI, Lijuan MIAO, Yuyou HE( 1. Jilin Agricultural Science and Technology College, Jilin 132101, China; 2. Viand Biotech Group, Qingdao 266000, China; 3. Jilin Zhengye Biology Product Ltd, Jilin 132101, China) |
| |
Abstract: | Objective] The aim of this study was to improve the purification and protective potency of HP-PRRS inactivated vaccine. Method] HP-PRRS virus that had been multiplied inside Marc-145 cells was collected and concentrated 50 times and then inactivated. Complete virions were separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified inactivated vaccine. Result] Viral protein was separated from other proteins by purification and the viral protein contents ranged from 76.7% to 82.4%, and 96% of the expected serum proteins were removed. Protective potency of purified vaccine was above 4/5 and positive conversion rate of antibody was over 86%, both higher than that of unpurified vaccine. The differences were significant. Conclusion] The experiment il-lustrated that the immune efficacy of vaccine can be enhanced through concentrat- ing and purifying, while the non-viral protein can be removed, so that allergic reaction and stress response cadsed by vaccine inoculation can be avoided. |
| |
Keywords: | HP-PRRS purified inactivated vaccine Protective potency Positive con-version rate of antibody Stress response |
本文献已被 CNKI 维普 等数据库收录! |
|