Construction of Highly-efficient Direct Regeneration System of Different Chrysanthemum Species |
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Authors: | Shijin XU Zhongchuan XU |
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Institution: | 1. Gold Mantis School of Architecture&Urban Environment, Soochow University, Suzhou 215123, China; 2. College of Biotechnology and Food Science, Changshu Enstitute of Technology, Changshu 215500, China |
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Abstract: | Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor- mone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species all showed higher regeneration capacity, with regeneration rate in 84%-100% and re- generation coefficient at 2.3-9.4. However, none adventitious buds were found differ- entiated from leaves of "28" and "5-17", and leaf regeneration capacity of "11" was lower, either. Most chrysanthemum species proved higher in stem regeneration ca- pacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering. |
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Keywords: | Dend-ranthema morifolium Leaf Stem Direct regeneration |
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