| 草樱花组培快繁技术的研究 |
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| 引用本文： | 王玉珍,徐进,罗景兰,史印山,刘香玲. 草樱花组培快繁技术的研究[J]. 山东农业科学, 2004, 0(6): 6-8 |
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| 作者姓名： | 王玉珍 徐进 罗景兰 史印山 刘香玲 |
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| 作者单位： | 中国科学院遗传与发育生物学研究所农业资源研究中心,河北,石家庄,050021;中国科学院遗传与发育生物学研究所农业资源研究中心,河北,石家庄,050021;中国科学院研究生院,北京,100039;河北省气象台,河北,石家庄,050021 |
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| 基金项目： | 中国科学院中欧观赏植物引进试验研究(05-47)项目资助。 |
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| 摘 要： | 以草樱花为材料,研究了激素配比、蔗糖、培养条件等组培苗工厂化生产关键技术。结果表明:采用MS+6-BA0 5mg/L+IBA0 05mg/L(或2,4-D0 25mg/L)+蔗糖30g/L用于分化培养,1/4MS+NAA0 5mg/L+蔗糖10g/L用于生根培养,光强1500～2000lx,光照时间每天12～14h条件下组培苗增殖系数最大,有效苗最多,生根率高及生长速度快,移栽成活率90%以上。采用管外扦插成活率80%以上。建立了草樱花工厂化生产工艺及试管苗培养标准,为其植物组培苗繁殖提供了理论依据。
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| 关 键 词： | 草樱花 组织培养 快速繁殖 |
| 文章编号： | 1001-4942(2004)06-0006-03 |
| 修稿时间： | 2004-09-20 |
Study on the technology of tissue culture and rapid multiplication of P.subulata L. |
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| WANG Yu-zhen,XU Jin. Study on the technology of tissue culture and rapid multiplication of P.subulata L.[J]. Shandong Agricultural Sciences, 2004, 0(6): 6-8 |
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| Authors: | WANG Yu-zhen XU Jin |
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| Affiliation: | WANG Yu-zhen~1,XU Jin~ |
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| Abstract: | Culturing the stem of P. subulata L. in vitro, the key techniques including medium,hormones,sucrose,culture conditions et al. were studied.The results showed that if there was MS+ 6-BA 0.5mg/L+ IBA 0.05mg/L(or 2,4-D 0.25mg/L)+ sucrose 30g/L for mutiplicating, 1/4MS +NAA 0.5mg/L+sucrose 10g/L for rooting,light intensity sustaining under 1500～2000lx and illuminated for 12～14h/d, the coefficient of multiplication,quantity of effective plantlets and rooting rate were best.The survival rate of transplantation was above (90%.)More than 80% of plantlets survived by using outer test-tube cuttage for rooting.Based on the study,the industrializing technology of micropagation of P.subulata L. by in vitro culture and the culture standard which improves a theoretical basic for micropagation of other plants were summarized. |
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| Keywords: | P.subulata L. tissue culture rapid propagation |
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