| 北柴胡鲨烯合酶基因及其编码区cDNA克隆与序列分析 |
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| 引用本文： | 隋春,魏建和,战晴晴,杨成民. 北柴胡鲨烯合酶基因及其编码区cDNA克隆与序列分析[J]. 园艺学报, 2010, 37(2): 283-290 |
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| 作者姓名： | 隋春 魏建和 战晴晴 杨成民 |
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| 作者单位： | 1. 中国医学科学院,北京协和医学院药用植物研究所,北京,100193
2. 中国医学科学院,北京协和医学院药用植物研究所,北京,100193;东北林业大学生命科学院,哈尔滨,150040 |
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| 基金项目： | 国家科技支撑计划重点项目，中央级公益性科研院所基本科研业务专项项目 |
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| 摘 要： | 鲨烯合酶(EC.2.5.1.21,squalene synthase,SS)是植物甾醇和三萜化合物生物合成途径中的关键酶。为研究鲨烯合酶在柴胡皂苷生物合成中的作用,以北柴胡(Bupleurum chinense DC.)不定根为试材,采用Trizol法提取总RNA,利用RT-PCR方法从北柴胡中扩增出了鲨烯合酶cDNA特异片段,并进行了克隆、测序。测序结果显示得到了两个序列不同的cDNA(BcSS1和BcSS2),分别为1245bp和1248bp,分别编码414及415个氨基酸。BcSS1和BcSS2间的核苷酸和氨基酸一致性分别为98%和96%。NCBI Blastx结果显示与三岛柴胡和三七SS氨基酸序列相似性最高,BcSS1的一致性分别为99%和90%,BcSS2的分别为97%和87%。在此基础上,利用PCR技术从北柴胡不定根中扩增得到了一个SS基因克隆,长5880bp,包含12个内含子。
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| 关 键 词： | 北柴胡 柴胡皂苷 鲨烯合酶基因（SS） RT-PCR PCR |
| 收稿时间： | 2009-11-26 |
| 修稿时间： | 2010-01-25 |
Cloning and Sequence Analysis of Squalene Synthase Gene and cDNA in Bupleurum chinense DC. |
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| SUI Chun,WEI Jian-he,ZHAN Qing-qing,,, YANG Cheng-min. Cloning and Sequence Analysis of Squalene Synthase Gene and cDNA in Bupleurum chinense DC.[J]. Acta Horticulturae Sinica, 2010, 37(2): 283-290 |
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| Authors: | SUI Chun WEI Jian-he ZHAN Qing-qing YANG Cheng-min |
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| Affiliation: | SUI Chun1,WEI Jian-he1,ZHAN Qing-qing1,2,, YANG Cheng-min1 (1Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China,2College of Life Science,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | Squalene synthase(EC.2.5.1.21,SS)is an important key enzyme in biosynthesis pathway of plant sterols and terpenoids.In order to learn the role of SS in the biosynthesis of saikosaponin in Bupleurum chinense DC.,SS cDNA was cloned with total RNA extracted from adventitious roots of B.chinense using Trizol method.Specific fragments were amplified by RT-PCR and then were cloned and sequenced.Sequencing results showed two different cDNA fragments(BcSS1 and BcSS2)with 1 245 bp and 1 248 bp long were obtained whi... |
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| Keywords: | RT-PCR PCR Bupleurum chinense DC. saikosaponin squalene synthase gene(SS) RT-PCR PCR |
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