海藻糖对盐胁迫下两个沙棘品种逆境生理的影响

摘要： 通过研究海藻糖对沙棘逆境生理指标的影响规律,探索海藻糖的抗盐生理机制,为生产中应用海藻糖缓解沙棘 盐胁迫提供理论依据 。试验在盆栽条件下,设置空白对照、盐胁迫 + 不同浓度的海藻糖(0、0.2、0.4、0.6 mmol·L-1 )共计 5个处理,3次重复 。结果表明:0.4 mmol·L-1 的海藻糖可以显著提高盐胁迫下两个沙棘品种叶片内的叶绿素、游离脯氨 酸含量,降低 MDA含量;海藻糖会提高盐胁迫下两个品种沙棘叶片内海藻糖-6-磷酸磷酸酶(TPP)活性,提高可溶性糖和可 溶性蛋白含量;0.4mmol·L-1 的海藻糖对提高 TPP活性效果优于0.2mmol·L-1和 0.6mmol·L-1处理,有利于可溶性糖的 积累,缓解盐胁迫对沙棘的危害 。海藻糖对 TPP活性的调控作用可能是其缓解沙棘盐胁迫的生理机制,0.4 mmol·L-1 为适 宜的喷施浓度。

EfectsofTrehaloseonStresPhysiologyofTwoHipophaerhamnoides VarietiesUnderSaltStres

Abstracts: ThisteststudiedtheefectofexogenoustrehaloseonthephysiologicalindicatorsofHipophaerhamnoides, andthephysiologicalmechanism oftrehaloseresistancetosaltwasexplored,whichitcanprovidea theoreticalbasisfor theapplicationoftrehaloseinproductiontoaleviatethesaltstres.Thetestunderpotedconditions,andthenset5treat- mentsincludingblankcontrol,saltstres+ diferentconcentrationsoftrehalose (0,0.2,0.4,0.6 mmol· L-l ),each treatmentwasrepeated3 times.Theresultsshowedthatthechlorophylandfreprolinecontentsintheleavesofthetwo H.rhamnoidesvarietiesof0.4 mmol·L-l trehalosewassignificantlyhigherthancontrolundersaltstres,andtheMDA contentwassignificantlylowerthancontrol;theactivityoftrehalose-6-phosphatephosphatase(TPP)intheleavesoftwo H.rhamnoidesvarietiesofexogenoustrehalosewashigherthancontrolundersaltstres,andthecontentofsolublesugar andsolubleproteinwassignificantlyhigherthancontrol;theefectof0.4 mmol·L-l exogenoustrehaloseonimproving TPPactivitywasbeterthanthatof0.2mmol·L-l and0.4mmol·L-l treatments,whichwasbeneficialtotheacumu- lationofsolublesugarandaleviatedtheharmofsaltstrestoH.rhamnoides.Theregulationefectofexogenoustreha- loseonTPPactivitymaybeitsphysiologicalmechanismtoaleviatethesaltstresofseabuckthorn,and0.4mmol·L-l is theappropriatesprayconcentration