A modified Murashige and Skoog media for efficient multipleshoot induction in G. arborea Roxb.

This paper reports on the effect of various micropropagation factors of Gmelina arborea Roxb. through multiple shoot induction. Factors like the source and age of explants, plant growth regulators (PGRs), media composition, and carbon source affected multiple shooting in the present study. Among all the explants used, only shoot tips derived from one, two, and three week old seedlings could form multiple shoots. Besides, the formation of multiple shoots depended on the concentration and combination of PGRs. Among all the PGRs, BAP (6-benzylaminopurine) alone gave the highest regeneration efficiency. Similarly, IBA (Indole-3-butyric acid) was the most efficient PGR in inducing root formation in the microshoots. Media composition and carbon source also affected the regeneration efficiency. MS (Murashige and Skoog medium) proved to be the best media for regeneration followed by B5, SH (Schenk and Hilderbrandt medium) and WPM (Woody plant medium) in that order. Similarly, among sugars, only sucrose and glucose supported induction of microshoots. Based on this study we recommend the use of glucose in place of sucrose in MS medium for maximum regeneration efficiency.