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三河牛ABCG2基因Y367C位点与产奶性状的关联分析
引用本文:卢振峰,秦春华,杨艳,刘莉莉,俞英,吴宏军,刘爱荣,王雅春,史远刚. 三河牛ABCG2基因Y367C位点与产奶性状的关联分析[J]. 中国牛业科学, 2013, 39(2): 24-26,30
作者姓名:卢振峰  秦春华  杨艳  刘莉莉  俞英  吴宏军  刘爱荣  王雅春  史远刚
作者单位:1. 中国农业大学动物科技学院,中国北京1001931;宁夏大学农学院,宁夏银川750021
2. 中国农业大学动物科技学院,中国北京,1001931
3. 北京市园林学校,中国北京,102488
4. 内蒙古谢尔塔拉种牛场,内蒙古海拉尔,021008
5. 内蒙古自治区海拉尔农垦集团有限公司,内蒙古海拉尔,021008
6. 宁夏大学农学院,宁夏银川,750021
基金项目:现代农业(奶牛)产业技术体系建设项目,"十二五"国家科技支撑计划,国家自然基金项目
摘    要:[目的]旨在检测三磷酸腺苷结合转运蛋白G超家族成员2(ABCG2)基因Y367C位点此在三河牛群体中的多态性,并分析了其与产奶性状之间的关系。[方法]利用Sequen—ornMassARRAYGenotype分析技术对该位点进行检测,通过SAS软件(9.1)对6个产奶性状与该位点的关联程度进行了统计分析。[结果]表明,Y367C位点在三河牛中均处于Hardy-Weinberg平衡状态(P〈0.05);ABCG2基因Y367C位点的YY基因型个体体细胞评分最低,CC个体产奶量、乳脂量、乳蛋白量最高;Y等位基因降低体细胞评分,提高乳蛋白率;而C等位基因则有助于提高产奶量、乳脂率、乳脂量和乳蛋白量,但三种基因型之间差异不显著(P〈0.05)。[结论]该位点可以为三河牛的分子育种提供参考。

关 键 词:三河牛  ABCG2基因  产奶性状  关联分析

Association of Y367C in ABCG2 Gene with Milk Production Traits in Sanhe Cattle
Affiliation:LU Zhen-feng,QIN Chun-huan,Yang Yan ,LIU Li-li ,YU WANG Ya-chun, SHI Yuan (1. College of Animal Science and Technology, China Agricultural University ,Beijing 100193 ;2. College of Agronomy, Ningxia University ,Yinchuan , Ning:ria 750021 ;3. Beijing Landscape Architecture School, Beijing 1024884. Inner Mongolia Hailaer Agricultural Reclamation Group co. ,L TD , Hailaer , Inner Mongolia 021008 ; 5. Inner Mongolia Xieertala Bull Station, Hailaer , Xieertala 021008)
Abstract:[Objective]The aim of this study is to detect Y367C site in ATP-bind ly G member [Method]The 2 (ABCG2) gene in Sanhe cattle and analyze its association with mg rail cassette super-fami- k production traits. detection of the site was analyzed with Sequenom Mass ARRAY Genotype technology. The association between the site and 6 production traits was analyzed with software SAS (version 9 [Result]The results showed that Y367C conformed to Hardy-Weinberg Law(P(0.05). Individuals 1). with YY genotype of Y367C site had the lowest somatic cell score while those with CC genotype had the high est milk yield, fat yield,and protein yield. Allele Y could improve somatic cell score and protein percentage. Allele C could improve milk yield,fat percentage, fat yield and protein yield, but there was no signifi cant difference among three genotypes(P〈0.05). [Conclusion]The site could provide reference for molec ular breeding in Sanhe cattle.
Keywords:Sanhe cattle  ATP-binding cassette super-family G member 2 (ABCG2)  milk productiontraits
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