| 金线莲组培快繁技术体系的研究 |
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| 引用本文： | 李艳冬,叶红霞,陈丽萍. 金线莲组培快繁技术体系的研究[J]. 江西农业学报, 2013, 25(4): 52-54,62 |
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| 作者姓名： | 李艳冬 叶红霞 陈丽萍 |
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| 作者单位： | 湖州市农业科学研究院,浙江湖州,313000 |
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| 基金项目： | 浙江省公益技术研究农业项目"药用植物金线莲种苗规模化生产技术研究"，浙江省湖州市一般科研项目"药用植物金线莲规模化生产技术研究" |
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| 摘 要： | 通过设置不同的试验来优化金线莲的组培条件,结果表明:金线莲外植体消毒处理以75%乙醇(浸泡30 s)+0.1%HgCl(浸泡8 min)的效果较好,诱导芽培养基以MS+BA 2.0 mg/L+NAA 0.3 mg/L的处理效果最优,芽丛生增殖培养基以MS+BA 1.5 mg/L+NAA 0.5 mg/L的效果最优,在增殖培养基中添加20%香蕉泥对芽苗的增殖及壮苗培养均有极大的促进作用。在芽体分化培养过程中,在MS+BA 0.05 mg/L+NAA 0.5 mg/L的培养基中,接种密度为每瓶10颗和800lx的光照处理效果较为理想。
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| 关 键 词： | 金线莲 茎段 组培快繁 芽丛生 芽诱导 |
Study on Tissue Culture and Rapid Propagation Techniques of Anoectochilus roxburghii |
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| LI Yan-dong , YE Hong-xia , CHEN Li-ping. Study on Tissue Culture and Rapid Propagation Techniques of Anoectochilus roxburghii[J]. Acta Agriculturae Jiangxi, 2013, 25(4): 52-54,62 |
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| Authors: | LI Yan-dong YE Hong-xia CHEN Li-ping |
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| Affiliation: | (Agricultural Sciences Research Institute of Huzhou City in Zhejiang Province,Huzhou 313000,China) |
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| Abstract: | Different tests were conducted to optimize the tissue culture conditions of Anoectochilus roxburghii.The results showed that 75% ethanol(soaking for 30 seconds) + 0.1% HgCl(soaking for 8 minutes) had better disinfection effect on the explants.The best medium for the bud inducement was MS+BA 2.0 mg/L+NAA 0.3 mg/L,while the best medium for the bud proliferation was MS+BA 1.5 mg/L+NAA 0.5 mg/L.Addition of 20% banana puree in the proliferation medium could improve bud proliferation and culture of strong seedlings.In the culture process of bud differentiation and the culture medium of MS+BA 0.05 mg/L+NAA 0.5 mg/L,the inoculum density of 10 plants per bottle and the light treatment of 800 lx could produce ideal effects. |
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| Keywords: | Anoectochilus roxburghii Stem segment Tissue culture and rapid propagation Bud proliferation Bud inducement |
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