| 一株产纤维素酶枸杞内生菌的分离鉴定 |
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| 引用本文： | 徐海燕,辛国芹,陈梅楠,谷巍.一株产纤维素酶枸杞内生菌的分离鉴定[J].江西农业学报,2013,25(4):38-42. |
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| 作者姓名： | 徐海燕 辛国芹 陈梅楠 谷巍 |
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| 作者单位： | 1. 山东宝来利来生物工程股份有限公司,山东泰安,271000
2. 山东省泰安市植物保护站,山东泰安,271000 |
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| 摘 要： | 通过组织分离法从健康枸杞植株体内分离到10株内生菌,经反复筛选及刚果红染色鉴定,获得一株较高活力纤维素酶内生菌GQP-3。结合菌落形态、生理生化特性及菌株特异性序列分析等手段,确定其分类归属。结果表明GQP-3为类芽孢杆菌属,该菌的发酵液在中性条件下的CMC酶活力可达20.0 U/mL。
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| 关 键 词： | 枸杞 纤维素酶 内生菌 |
Isolation and Identification of An Endogenetic Strain Producing Cellulase from Wolfberry |
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| XU Hai-yan
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XIN Guo-qin
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CHEN Mei-nan
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GU Wei.Isolation and Identification of An Endogenetic Strain Producing Cellulase from Wolfberry[J].Acta Agriculturae Jiangxi,2013,25(4):38-42. |
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| Authors: | XU Hai-yan XIN Guo-qin CHEN Mei-nan GU Wei |
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| Institution: | 1(1.Shandong Baolai-Leelai Bioengineering Company Limited,Tai’an 271000,China; 2.Tai’an City Plant Protection Station of Shandong Province,Tai’an 271000,China) |
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| Abstract: | Ten endogenetic strains were isolated from healthy wolfberry plants by tissue isolation.Among them,the strain GQP-3 was the endogenetic strain producing cellulase with higher vigor,and it was obtained by repeated screening and Congo red dying method.In order to classify this strain,some means were used,such as colonial morphology,physiological and biochemical characteristics,strain-specific sequence analysis and so on.The results revealed that the strain GQP-3 was identified as Paenibacillus,and CMC enzyme activity of fermentation broth of the strain in the neutral condition could reach 20.0 U/mL. |
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| Keywords: | Wolfberry Cellulase Endogenetic strain |
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