棉花γ射线诱变后代的SSR标记遗传多样性

利用39个多态性SSR标记分析了三个棉花品种辐射诱变后代M5材料（共74份）的遗传多样性。结果表明，三个品种各检测到的多态性位点占总个多态性位点的百分数分别为51.3%、48.7%和66.7%；且其基因型多样性（H''）的平均值分别为0.997、0.648、1.187。三个品种诱变后代材料与其未辐射处理对照的遗传相似系数变幅分别为0.6515～0.9697、0.6970～1.0000、0.5524～0.8095，其平均遗传相似系数分别为0.8637、0.9206、0.6818。Arcot-1和Su9108两个品种诱变后代的成对相似系数大于或等于8.000的比例达到60.0%以上，J11诱变后代的成对相似系数主要集中在低相似性区域，其对成相似系数小于7.000的比例达62.5%。该研究从分子水平揭示出辐射可创造丰富的遗传变异，拓宽了原品种的遗传基础。同时还明确了辐射对不同棉花品种的诱变效果的差异，为辐射诱变创新种质提供了可靠的检测方法和理论基础。

Genetic Diversity of the Progeny of Cotton (Gossypium hirsutum L.)Irradiated by γ Ray with SSR Markers

The genetic diversity of 74 mutagenic progenies M5 of 3 cotton varieties, Arcot-1, Su9108 and J11, irradiated by γ-ray were analyzed with 39 polymorphism SSR primer pairs. The results are as follows. Polymorphic loci of SSR markers which can detected among the mutagenic progeny M5 of 3 cotton varieties accounted for 51.3%, 48.7% and 66.7% to the total respectively, and the averages of diversity indexes (H') among the mutagenic progeny M5 of 3 cotton varieties were 0.997, 0.648, 1.187 respectively. The variance range of genetic similarity coefficient of SSR markers among the mutagenic progeny M5 of 3 cotton varieties and its original CK were 0.6515～0.9697，0.6970～1.000，0.5524～08095 respectively, and the averages were 0.8637, 0.9206, 0.6818. The pair similarity coefficient that was higher or equal to 8.000 was more than 60.0% for the mutagenic progenies M5 of two varieties (Arcot-1 and Su9108). The pair similarity coefficient that less than 7.000, was 62.5% for the mutagenic progenies M5 of J11. The analysis of molecular markers indicated that abundant genetic variation can result and genetic base in original varieties can be broadened from theγ-ray radiation. The study also clarified that the radiation of different cotton varieties resulted in the different variation, and provided detective method and theoretic base for germplasm enhancement by usingγ-ray radiation.