Effect of booster shot and investigation of vaccination efficacy period against herpesviral haematopoietic necrosis (HVHN) in goldfish Carassius auratus |
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| Institution: | 1. Tamaki Laboratory, Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruda, Tamaki, Mie 519-0423, Japan;2. Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-Ise, Mie 516-0193, Japan;1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, PR China;2. Research Division, Clear Springs Foods, Inc., PO Box 712, Buhl, ID 83316, USA;3. Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA;1. College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, PR China;2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, PR China;1. School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China;2. Aquaculture Technical Extension Station of Dafeng City, Dafeng 224100, China;3. Agricultural Biotechnology Research Institute, Agricultural biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China;1. Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China;2. Baoying Center for Control and Prevention of Aquatic Animal Infectious Disease, 30# Yeting East Road, Baoying, 225800, China;3. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu, 222005, China;1. ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751 002, India;2. Peninsular and Marine Fish Genetic Resources Centre of ICAR-National Bureau of Fish Genetic Resources, CMFRI Campus, Ernakulum North PO, Kochi 682 018, India;3. Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata 700 094, India;4. ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, Dilkusha, Lucknow 226 002, India;1. College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430071, China;2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, China |
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| Abstract: | In this study, the efficacy period of an intraperitoneal vaccination and effect of a booster shot of vaccine against herpesviral haematopoietic necrosis (HVHN) in goldfish Carassius auratus were investigated. Cell culture supernatant of cyprinid herpesvirus 2 (CyHV-2), causative agent of HVHN, propagated in goldfish fin (GFF) cells was inactivated with formalin (0.1%, v/v) for 2 days at 4 °C. Three groups of the variety Ryukin were individually intraperitoneally injected with the vaccine and each group was separately maintained in replicate tanks. After 4 weeks (Vaccinated-4w-1 and 2) and 8 weeks (Vaccinated-8w-1 and 2) from the first vaccination, the fish were CyHV-2-challenged by the immersion route (10 TCID50 l?1). In addition, the other vaccinated group of fish were injected with a booster vaccine 4 weeks after the first vaccination as the Vaccinated-booster groups, then the fish of these groups were CyHV-2-challenged by the immersion route (10 TCID50 l?1) after 8 weeks from the first vaccination. The mean of the relative percentage survival (RPS) values of the Vaccinated-4w and 8w groups showed 42.5% and 57.6%, respectively. In addition, the mean RPS value of Vaccinated-booster groups showed 63.6%. Statistical analysis showed significantly higher survival rates in all the vaccinated groups than those of the respective negative control groups using Fisher's exact test. Moreover, the survival rates of vaccinated-booster groups were significantly higher (p = 0.036) compared with the respective control groups by Student's t test. The present study shows the efficacy period of the vaccine is at least 8 weeks and a booster shot showed a tendency to enhance the protection against HVHN in goldfish. |
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| Keywords: | Fish viral disease Goldfish Herpesviral haematopoietic necrosis Cyprinid herpesvirus 2 Vaccination |
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