小檗碱和大蒜素对香榧绿藻抑藻作用研究

本文探讨了小檗碱对香榧绿藻生长的抑制机制。验证大蒜素对香榧绿藻的抑制效应,比较小檗碱和大蒜素的抑藻效果。通过共培养实验并测定香榧绿藻的藻细胞密度、叶绿素a含量、SOD活性、CAT活性、MDA含量来分析小檗碱的抑藻效果及抑藻机制；通过混合正交实验,研究了大蒜素、槐糖脂助剂、培养时间三因素对香榧绿藻抑制效果的影响。小檗碱的抑制效果与浓度和培养时间呈正相关；最高抑藻率为85.45%(12 d,320 μg/mL)。叶绿素a含量变化与藻细胞密度趋势一致。SOD、CAT活性均先升后降；MDA含量较对照组持续升高。大蒜素的混合正交实验结果表明最佳组合为大蒜素浓度320 μg/mL、槐糖脂助剂浓度20 μg/mL、培养12 d,抑制效果最高可达85.65%。以上研究结果表明小檗碱对香榧绿藻的抑制作用存在低促高抑现象。大蒜素有望制备成一种新型的杀藻剂,配合槐糖脂助剂使用,抑藻率略高于小檗碱。

Study on the anti-algae effect of berberine and allicin of the algae on Torreya grandis

The purpose of this study was to investigate the inhibitory mechanism by which berberine suppressed the proliferation of Chlorella sp. on Torreya grandis, to verify the inhibitory effect of allicin on Chlorella sp. on Torreya grandis. and to compare the efficacy of two types of plant-derived substances in inhibiting the growth of Chlorella sp. on Torreya grandis Through co-