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Fine mapping and conservation analysis of linear B-cell epitopes of peste des petits ruminants virus nucleoprotein
Institution:1. Institute of Animal Science and Veterinary Medicine, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201106, China;2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China;3. Department of Reproductive Biology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China;1. Division of Health Sciences, University of Warwick Medical School, Coventry, United Kingdom;2. National Institute for Health Research, Collaboration for Leadership in Applied Health Research and Care (CLAHRC), University of Warwick Medical School, Coventry, United Kingdom;3. Department of Mathematics and Information Sciences, Faculty of Engineering and Environment, Northumbria University, Newcastle upon Tyne, United Kingdom;4. Department of Population Health, Luxembourg Institute of Health, Luxembourg;5. Warwick Center for Applied Health Research and Delivery, Division of Health Sciences, University of Warwick Medical School, Coventry, United Kingdom;6. Liverpool School of Tropical Medicine, International Health Group, Liverpool, United Kingdom;1. Research and Development, MCI Santé Animale, Lot. 157, Z. I., Sud-Ouest (ERAC) B.P.: 278, Mohammedia 28810, Morocco;2. The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, UK;3. Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco;4. IWK Health Centre, Canadian Center for Vaccinology, Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada;1. Institut National de Médecine Vétérinaire (INMV), Cinq Maison, El Harrach, Algiers, Algeria;2. Laboratoire de Recherche “Santé et Productions Animales”, Ecole Nationale Supérieure Vétérinaire d''Alger, El Alia, Algiers, Algeria;3. Applied Biotechnology Division, National Veterinary Research Institute, PMB 1, Vom, Plateau State, Nigeria
Abstract:Nucleoprotein (NP) is the most abundant and highly immunogenic protein of morbillivirus, and is presently the basis of most diagnostic assays for peste des petits ruminants virus (PPRV). In this study, fine epitope mapping and conservation analysis of linear B-cell epitopes on the PPRV NP has been undertaken using biosynthetic peptides. Nineteen linear B-cell epitopes were identified and their corresponding minimal motifs were located on the NP of PPRV China/Tibet/Geg/07-30. Conservation analysis indicated that ten of the 19 minimal motifs were conserved among 46 PPRV strains. Peptides containing the minimal motifs were recognized using anti-PPRV serum from a goat immunized with PPRV vaccine strain Nigeria 75/1. Identified epitopes and their motifs improve our understanding of the antigenic characteristics of PPRV NP and provide a basis for the development of epitope-based diagnostic assays.
Keywords:Peste des petits ruminants virus  Nucleoprotein  Linear epitope  Minimal motif  Mapping
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