Changes in peripheral blood leucocytes of sheep experimentally infected with Mycoplasma agalactiae |
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| Institution: | 1. Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;2. Department of Veterinary Medicine, Università degli Studi di Bari, Strada P.le per Casamassima Km 3, Valenzano, Bari 70010, Italy;1. Mestrado em Ciência Animal, Universidade Estadual do Maranhão, São Luís, MA, Brazil;2. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;3. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;1. Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands;2. Department of Gerontology and Geriatrics, Leiden University Medical Center, Leiden, The Netherlands;3. Leyden Academy on Vitality and Ageing, Leiden, The Netherlands;1. Department of Chemistry, Ferdowsi University of Mashhad, 917751436 Mashhad, Islamic Republic of Iran;2. Departament de Química, Universitat de les Illes Balears, Crta. de Valldemossa km 7.5, 07122 Palma de Mallorca (Baleares), Spain;3. Department of Chemistry, Tulane University, New Orleans, LA 70118, USA |
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| Abstract: | Contagious agalactia is a serious disease of small ruminants affecting mainly mammary glands, joints and eyes. In sheep, the main aetiological agent is Mycoplasma agalactiae (Ma) whose abilities to persist in the target organs are known. Since there is no information on the effect of acute and chronic Ma infection on circulating leucocytes, the present study was designed to monitor granulocytes, monocytes, T and B lymphocytes, by flow cytometry, in female lactating sheep nasally infected with Ma. A profound depletion of leucocytes was observed from day 5 to day 34 post infection (p.i.). In particular, while the granulocytes returned to baseline levels by day 12 p.i., the monocytes remained significantly low until day 20 p.i. The infection caused a prolonged depletion of peripheral T lymphocytes (both CD4+ and CD8+) while B lymphocytes remained unaltered throughout the study. Mycoplasma agalactiae was detected by real-time PCR in several anatomical sites (ear, nose and milk) from day 2–5 p.i. until the end of the study (i.e., day 50 p.i.) while a transient bacteraemia was observed from day 5 to day 12 p.i. The leucopenia observed following intranasal Ma infection is likely due to leucocyte infiltration within the target organs. |
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| Keywords: | Mycoplasma agalactiae Granulocytopenia Monocytopenia Lymphopenia |
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