The genetic organization of the capsular polysaccharide biosynthesis region
of Actinobacillus pleuropneumoniae serotype 15 |
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| Authors: | Hiroya ITO Masuo SUEYOSHI |
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| Affiliation: | 1)National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1–5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan;2)Center for Animal Disease Control, University of Miyazaki, 1–1 Gakuen-Kibanadai, Miyazaki 889-2192, Japan |
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| Abstract: | Nucleotide sequence determination and analysis of the cps gene involvedin the capsular polysaccharide biosynthesis of Actinobacilluspleuropneumoniae serotype 15 revealed the presence of three open readingframes, designated as cps15ABC genes. At the protein level, Cps15A andCps15B showed considerably high homology to CpsA (67.0 to 68.7%) and CpsB (31.7 to 36.8%),respectively, of A. pleuropneumoniae serotypes 1, 4 and 12, revealing thecommon genetic organization of the cps among serotypes 1, 4, 12 and 15.However, Cps15C showed no homology to any proteins of A. pleuropneumoniaeserotypes, indicating that cps15C may be specific to serotype 15. Thisstudy will provide the basic molecular knowledge necessary for the development ofdiagnostics and a vaccine for A. pleuropneumoniae serotype 15. |
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| Keywords: | Actinobacillus pleuropneumoniae serotype 15 capsule |
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