The genetic organization of the capsular polysaccharide biosynthesis region
of Actinobacillus pleuropneumoniae serotype 15 |
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Authors: | Hiroya ITO Masuo SUEYOSHI |
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Institution: | 1)National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1–5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan;2)Center for Animal Disease Control, University of Miyazaki, 1–1 Gakuen-Kibanadai, Miyazaki 889-2192, Japan |
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Abstract: | Nucleotide sequence determination and analysis of the cps gene involved
in the capsular polysaccharide biosynthesis of Actinobacillus
pleuropneumoniae serotype 15 revealed the presence of three open reading
frames, designated as cps15ABC genes. At the protein level, Cps15A and
Cps15B showed considerably high homology to CpsA (67.0 to 68.7%) and CpsB (31.7 to 36.8%),
respectively, of A. pleuropneumoniae serotypes 1, 4 and 12, revealing the
common genetic organization of the cps among serotypes 1, 4, 12 and 15.
However, Cps15C showed no homology to any proteins of A. pleuropneumoniae
serotypes, indicating that cps15C may be specific to serotype 15. This
study will provide the basic molecular knowledge necessary for the development of
diagnostics and a vaccine for A. pleuropneumoniae serotype 15. |
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Keywords: | Actinobacillus pleuropneumoniae serotype 15 capsule |
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