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Identification of phytotoxins from <Emphasis Type="Italic">Botryosphaeria obtusa</Emphasis>, a pathogen of black dead arm disease of grapevine
Authors:Jules Désiré Djoukeng  Suzanna Polli  Philippe Larignon  Eliane Abou-Mansour
Institution:1.Institute of Biology,University of Neuchatel,Neuchatel,Switzerland;2.Institut Fran?ais de la Vigne et du Vin, Unité Rh?ne-Méditerranée,Domaine de Donadille,Rodilhan,France;3.Plant Biology Department,University of Fribourg,Fribourg,Switzerland
Abstract:A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ -resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR.
Keywords:Black dead arm            Botryosphaeria obtusa            Grapevine disease  Isocoumarins  Melleins  Phytotoxins
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