兽用吡喹酮-聚乳酸-羟基乙酸缓释植入棒含量测定及释放度考察

建立了吡喹酮-聚乳酸-羟基乙酸（吡喹酮-PLGA）缓释植入棒含量测定的HPLC法，并对其释放度进行了考察。以甲醇-水（100：40，V／V）为流动相，采用GraceC18反相色谱柱（4．6mm250mm，5μm），流速1．0mL／min，紫外检测波长263nm，在此试验条件下，吡喹酮在10—200μg／mL范围内与峰面积线性关系良好，r=0．9999；平均回收率为99．48％，RSD为0．53％（n=9）。三批样品的含量分别为98．96％、98．67％和98．81％，该方法简单、快捷、辅料无干扰、准确度高，适于吡喹酮-PLGA植入棒的含量测定。吡喹酮-PLGA植入棒以骨架溶蚀释放机制缓慢释放，释药期可达三周，释放度高于300μg／d。

Determination and Release rate of Veterinary Sustained-release Praziquantel-PLGA Implants

The paper estabalisted an HPLC method for determination of the praziquantel - PLGA implants, and investigate release rate. The diamonsil C18 column was used, the mobile phase consisted of methanol and uhrafihrated water （100：40,v/v）, the flow rate was 1.0 mL/min, the detection wavelength was at 263 nm. Praziquanel has a good linearity at the range of 10 -200μg/mL（r =0. 9999） , and the average recovery was 99.48% with RSD O. 53% （ n = 9 ）. The determinations of three lots samples are 98. 96%, 98.67% and 98.81%, respectively. Praziquantel can be slowly released from the implant to three weeks and release rate is more than 300 μg/d. The method is simple, rapid and accurate without excipients interference, and suitable for the determination of the praziquantel - PLGA implants. Praziquantel can be slowly released with the frame - erosion mechanism.