Identification of a bisegmented double-stranded RNA virus (picobirnavirus) in calf faeces |
| |
Authors: | Buzinaro M G Freitas P P S Kisiellius J J Ueda M Jerez J A |
| |
Institution: | Departamento de Medicina Veterinária Preventiva e Reprodu??o Animal, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900 Jaboticabal, Estado de S?o Paulo, Brazil. gloriinha@fcav.unesp.br |
| |
Abstract: | To determine the incidence of rotavirus infection among dairy herds in the State of S?o Paulo, Brazil, 576 faecal samples obtained from calves aged 1-45 days with and without diarrhoea, reared on 63 dairy cattle farms, were analyzed. Polyacrylamide gel electrophoresis (PAGE) identified 28 samples positive for group A rotavirus, while four samples, two diarrhoeic and two non-diarrhoeic, showed a bisegmented genome with a typical picobirnavirus pattern. Electron microscopy revealed spherical virus particles with a diameter of 37 nm and without a defined surface structure. The present study is the first report of a bisegmented virus identified in cattle in Brazil. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|