反胶束中单宁酶催化没食子酸烷基酯的合成

没食子酸烷基酯是一类优良的抗氧化剂，具有潜在的药用价值。为了避免化学合成方法带来的弊端，建立了生物合成没食子酸烷基酯的方法。利用单宁酶在有机相可成功地催化没食子酸烷基酯的合成。在合成没食子酸丙酯(PG)、没食子酸丁酯(BG)和没食子酸戊酯(AG)的反胶束(reverse micelle, RM)催化合成体系中，反胶束组成为双(2-乙基己基)磺基琥珀酰钠(AOT)、异辛烷和水。单宁酶浓度为0.6 mg/mL；pH值为6； W0(水与表面活性剂的摩尔比)分别为15、15和12.5；反应温度分别为30、40和45℃；AOT浓度分别为0.1、0.2和0.2 mol/L时，没食子酸转化率分别为87.4%、90.3%和92.5%。3个体系中由于底物脂肪醇的不同，致使各种因素对单宁酶催化活力的影响不同。

Biosynthesis of Alky Gallates Catalyzed by Tannase in Reverse Micelle

Alky gallate is a kind of antioxidant and processes patient therapy value. However, it is synthesized by chemical method, which brings serious circumstance containment and low productivity. To avoid the defects, the method of biosynthesis for alky gallates was established in reversed micelles reaction system, in which propyl gallate (PG), butyl gallate (BG) and amyl gallate (AG) were successfully synthesized. The reverse micelle consisted of docusate sodium salt (AOT), isooctane and water; the tannase concentration was 0.6 mg/mL. In the reverse micelle reaction systems for PG, BG and AG, when W0 (the mole ratio of water to AOT) was respectively 15,15 and 12.5; reaction temperatures were 30, 40 and 45 ℃, respectively; AOT concentrations were 0.1, 0.2 and 0.2 mol/L respectively; pH values all were 6, conversion of gallic acid reached the highest in the different systems. The highest conversion rates of gallic acid reached 87.4%, 90.3% and 92.5% respectively. Various factors had different effects on tannase catalytic activity due to three different alky alcohol isomers.