转phyB基因枳橙生物学特性分析

对转phyB基因枳橙进行分子检测和部分生物学特性研究。PCR、RT-PCR和PCR Southern杂交结果表明:phyB基因已整合到枳橙的基因组,并得到了转录;转phyB基因枳橙表现出植株矮化、分枝角度加大、叶片变小、比叶干重增加,其叶绿素含量比对照高29.1%。对枳橙叶片光合特性分析结果表明,转phyB基因枳橙光合作用增强,其净光合速率对有效辐射强度和CO2浓度响应值显著高于对照,对强光和高浓度CO2的利用能力显著高于对照。利用高效液相色谱法测定转phyB基因枳橙叶片的内源激素含量,结果表明,其GA1、GA4、ABA、Z分别比对照高32.7%、50%、121%、17.8%,而IAA的含量比对照低,生长类激素与抑制类激素的比值差异显著,(GA1+GA4)/ABA,Z/ABA分别比对照低38.4%和43.2%。

Biological Characteristics of phyB Gene-based Transgenic Citrange Plants

Citranges transferred with phyB gene were detected at the molecular level and their biological characteristics were analyzed. PCR, RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange. Compared with the non-transgenic plant, the transgenic plant was dwarf, with larger shoot insertion angle and smaller leaf area, and was higher in chlorophyll content, net photosynthesis rate, light saturation point and specific leaf weight. Determination of endogenous hormone by HPLC indicated that the content of GA1, GA4, ABA and Z of the transgenic plant was 32.7%, 50%, 121% and 17.8% higher than that of the non-transgenic plant, respectively, whereas the content of IAA was lower than that of the non-transgenic plant. (GA1+ GA4)/ABA and Z/ABA ratios of the transgenic plant were 38.4% and 43.2% lower than those of the control, respectively.