辣椒MLO基因家族鉴定及表达分析

MLO基因是植物中特有的一类抗病负调控因子,该基因突变导致植物产生广谱抗病性。运用生物信息学方法,在辣椒基因组中鉴定出17条候选MLO基因序列。其分子量范围187~593kDa,等电点范围6.65~9.7。结构分析发现,辣椒MLO基因具有较多内含子,并且差异性较大。发现辣椒MLO基因有20个基序,分布较均匀且排列顺序一致。进化分析发现,单子叶和双子叶MLO基因在进化上有差异,与辣椒关系最近的是茄科的番茄。表达分析显示,辣椒MLO基因具有表达特异性。Capana01g003047在脱落酸诱导下,显著上调表达。Capana05g002411对脱落酸和疫病具有抵抗作用,在其诱导下上调表达。Capana08g000223同样在疫病胁迫下上调表达。

Genome-wide Identification and Expression Analysis of MLO Gene Family in Capsicum annuum

MLO gene is a special kind of negative control factor of disease resistance in plants. The gene mutation causes broad-spectrum resistance of plants. Using bioinformatics, 17 candidate MLO gene sequences were identified in the pepper genome. The range of molecular weight is 187~593kDa, and the range of isoelectric point is 6.65~9.7. Structural analysis showed that the MLO gene of pepper had more introns and had great difference. It was found that the MLO gene of pepper had 20 motifs, and the distribution of the motifs was uniform and the order of arrangement was consistent. Evolutionary analysis showed that the MLO genes of monocotyledon and dicotyledonum were different in evolution, and the closest relationship with capsicum was tomato in the Solanaceae. The expression analysis showed that the MLO genes of Capsicum had a specific expression. The expression of Capana01g003047 was significantly up-regulated by abscisic acid. Capana05g002411 was resistant to abscisic acid and epidemic disease, and was up-regulated under their induction. Capana08g000223 was also up-regulated under the stress of epidemic disease.