活血散结方含药血浆对视网膜色素上皮细胞毒性作用的研究

目的 制备活血散结方含药血浆，并研究其不同浓度对视网膜色素上皮（retinal pigment epithelium，RPE）细胞的毒性作用。方法 以成年健康家兔为对象，制备活血散结方含药血浆。将提取的兔原代RPE细胞分9个组，分别为：空白对照组、正常血浆组、5%含药血浆组、10%含药血浆组、20%含药血浆组、40%含药血浆组、60%含药血浆组、80%含药血浆组、100%含药血浆组，予以相对应浓度的含药血浆干预，CCK8法检测含药血浆对兔RPE细胞毒作用。结果 活血散结方各浓度含药血浆对RPE细胞有抑制作用，其中5%~20%含药血浆对RPE细胞有较弱的抑制作用，但与正常血浆比较，抑制率差异无统计学意义（P>0.05）；而40%~100%含药血浆对RPE细胞生长有明显的抑制作用，与正常血浆比较，抑制率差异有统计学意义（P<0.05）。结论 活血散结方含药血浆对RPE细胞有一定的抑制作用，抑制程度与含药血浆浓度成量效关系，可以考虑选择5%~20%含药血浆浓度作为后续实验研究的干预浓度。

A Study of the Toxic Effect of Huoxue Sanjie Decoction-Containing Plasma on Retinal Pigment Epithelium Cells

Objective To prepare Huoxue Sanjie Decoction-containing plasma, and to study the toxic effect of different concentrations of the plasma on retinal pigment epithelium (RPE) cells. Methods Adult healthy rabbits were used to prepare Huoxue Sanjie Decoction-containing plasma. Rabbit primary cells were divided into nine groups: blank control group, normal plasma group, 5% drug-containing plasma group, 10% drug-containing plasma group, 20% drug-containing plasma group, 40% drug-containing plasma group, 60% drug-containing plasma group, 80% drug-containing plasma group, and 100% drug-containing plasma group. These groups were treated with respective concentrations of drug-containing plasma. CCK-8 assay was used to examine their cytotoxic effect on rabbit RPE cells. Results All concentrations of Huoxue Sanjie Decoction-containing plasma had an inhibitory effect on RPE cells. The 5%, 10%, and 20% drug-containing plasma had a weak inhibitory effect on RPE cells, which was not significantly different from the inhibitory effect of normal plasma (P>0.05). However, the 40%, 60%, 80%, and 100% drug-containing plasma had an obvious inhibitory effect on RPE cell growth, and the inhibition rates were significantly from that of the normal plasma group (P<0.05). Conclusion Huoxue Sanjie Decoction-containing plasma has a certain inhibitory effect on RPE cells, which is correlated with the concentration of drug-containing plasma. The 5%-10% concentration of drug-containing plasma can be considered as the intervention concentration in the following experimental studies.