大菱鲆脾肾结节病病原菌的分离和鉴定 Isolation and Identification of Mycobacterium marinum Associated with Splenic and Renal Granuloma Disease of Cultured Turbot (Scophthalmus maximus)期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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大菱鲆脾肾结节病病原菌的分离和鉴定

作者姓名：	李杰刘耀宽白露陈四清阎永伟莫照兰

作者单位：	中国水产科学研究院黄海水产研究所农业农村部海水养殖病害防治重点实验室青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071;中国水产科学研究院黄海水产研究所农业农村部海水养殖病害防治重点实验室青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071;青岛农业大学海洋科学与工程学院青岛 266109;中国水产科学研究院黄海水产研究所农业农村部海水养殖病害防治重点实验室青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室青岛 266071;上海海洋大学水产与生命学院上海201306

基金项目：	国家重点研发计划(2018YFC0311300)、国家自然科学基金-山东省人民政府联合基金(U1706205)、中国水产科学研究院黄海水产研究所基本科研业务费(20603022017008)和鳌山科技创新计划(2015ASKJ02)共同资助

摘要：	2017年夏季，江苏省连云港市某养殖场大菱鲆出现大规模死亡，发病鱼体表无明显症状，解剖可见脾脏、肾脏出现白色散在结节，从发病鱼的内脏中分离得到1株优势菌SM-Myco001。人工感染实验结果显示，SM-Myco001可以引起大菱鲆内脏结节症状并造成鱼类死亡，且在高养殖水温(22℃)条件下发病更为剧烈。生理生化鉴定和16S rRNA基因分析结果显示，SM-Myco001属于海分枝杆菌(Mycobacterium marinum)。本研究首次报道了我国养殖大菱鲆感染海分枝杆菌的病例，可为大菱鲆养殖过程中的疾病防控提供参考。
关键词：	大菱鲆海分枝杆菌分枝杆菌病结节
收稿时间：	2018/7/21 0:00:00
修稿时间：	2018/9/3 0:00:00
Isolation and Identification of Mycobacterium marinum Associated with Splenic and Renal Granuloma Disease of Cultured Turbot (Scophthalmus maximus)

Authors:	LI Jie LIU Yaokuan BAI Lu CHEN Siqing YAN Yongwei and MO Zhaolan

Institution:	Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071; Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071 and Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306

Abstract:

Keywords:	Scophthalmus maximus Mycobacterium marinum Mycobacteriosis Granuloma
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