| 引起葡萄叶斑病的链格孢种类的初步鉴定 |
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| 引用本文： | 王彩霞,李兴红,魏艳敏,燕继晔,王勇,张玮.引起葡萄叶斑病的链格孢种类的初步鉴定[J].植物保护学报,2019,46(1):175-183. |
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| 作者姓名： | 王彩霞 李兴红 魏艳敏 燕继晔 王勇 张玮 |
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| 作者单位： | 北京农学院植物科学技术学院, 北京 102206;北京市农林科学院植物保护环境保护研究所, 北方果树病虫害绿色防控北京市重点实验室;农业部华北都市农业重点实验室, 北京 100097,北京市农林科学院植物保护环境保护研究所, 北方果树病虫害绿色防控北京市重点实验室;农业部华北都市农业重点实验室, 北京 100097,北京农学院植物科学技术学院, 北京 102206,北京市农林科学院植物保护环境保护研究所, 北方果树病虫害绿色防控北京市重点实验室;农业部华北都市农业重点实验室, 北京 100097,贵州大学农学院, 贵阳 550025,北京市农林科学院植物保护环境保护研究所, 北方果树病虫害绿色防控北京市重点实验室;农业部华北都市农业重点实验室, 北京 100097 |
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| 基金项目： | 国家葡萄产业技术体系专项（CARS-29-bc-2） |
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| 摘 要： | 为明确北京市大兴区、顺义区和山西省襄汾县葡萄树上叶斑病的病原菌,利用组织分离法对病样进行分离纯化,采用多基因系统发育学分析(ITS,Alt a 1和gpd)和形态学对纯化后菌株进行鉴定,并结合柯赫氏法则对其进行验证。结果表明,共获得37株链格孢属真菌菌株,分属链格孢Alternaria alternata、葡萄链格孢A. viniferae和乔木链格孢A. arborescens,柯赫氏法则检测结果显示接种菌株对所接叶片与果穗均具致病性,说明这3种链格孢种均为葡萄叶斑病的病原菌;葡萄链格孢为主要的病原菌,有28株,故将该类病害命名为葡萄链格孢叶斑病;此外,该类菌株在2～40℃之间均能生长,25℃为最适生长温度。
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| 关 键 词： | 葡萄 叶斑病 链格孢属 病原菌鉴定 |
| 收稿时间： | 2017/8/29 0:00:00 |
Identification of Alternaria species causing leaf spots in grapes |
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| Wang Caixi,Li Xinghong,Wei Yanmin,Yan Jiye,Wang Yong and Zhang Wei.Identification of Alternaria species causing leaf spots in grapes[J].Acta Phytophylacica Sinica,2019,46(1):175-183. |
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| Authors: | Wang Caixi Li Xinghong Wei Yanmin Yan Jiye Wang Yong and Zhang Wei |
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| Institution: | College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China;Key Laboratory of Urban Agriculture(North China), Ministry of Agriculture;Beijing Municipal Key Laboratory for Environmental Friendly Management on Pests of North China Fruits, Institute of Plant and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China,Key Laboratory of Urban Agriculture(North China), Ministry of Agriculture;Beijing Municipal Key Laboratory for Environmental Friendly Management on Pests of North China Fruits, Institute of Plant and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China,College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China,Key Laboratory of Urban Agriculture(North China), Ministry of Agriculture;Beijing Municipal Key Laboratory for Environmental Friendly Management on Pests of North China Fruits, Institute of Plant and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China,College of Agriculture, Guizhou University, Guiyang 550025, Guizhou Province, China and Key Laboratory of Urban Agriculture(North China), Ministry of Agriculture;Beijing Municipal Key Laboratory for Environmental Friendly Management on Pests of North China Fruits, Institute of Plant and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China |
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| Abstract: | |
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| Keywords: | grape leaf spot Alternaria pathogen identification |
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