Identification of terpenoids volatilized from Thymus vulgaris L. by heat treatment and their in vitro antimicrobial activity |
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| Affiliation: | 1. Field Science Center, College of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki, 300-0331, Japan;2. Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia;3. College of Agriculture, Ibaraki University, Chuo, Ami, Inashiki, Ibaraki, 300-0393, Japan;1. Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland;2. Department of Food Chemistry and Instrumental Analysis, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland;1. Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156 83111, Iran;2. Centro de Estudos Do Ambiente e Do Mar Lisboa, Faculdade de Ciências, Universidade de Lisboa, CBV, DBV, 1749-016, Lisboa, Portugal;1. INBIAS-CONICET, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina;2. IMICO CONICET, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Córdoba, Argentina;3. IMICO-CONICET, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina;1. Crop Research Institute, Drnovska 507, 161 06 Prague 6, Czech Republic;2. Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic |
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| Abstract: | Enhanced antifungal activity by hot water treatment at 50 °C 20 s against Botrytis cinerea Pers. ex Fr., a gray mold pathogen was evaluated in thyme (Thymus vulgaris L.) Thymol, p-cymene, and γ-terpinene were detected as the main volatile compounds in thyme plants. Emission of p-cymene and γ-terpinene from thyme increased after treatment, whereas amount of thymol released did not change, suggested that existence of a thermo-dependent synthesis pathway of p-cymene and γ-terpinene. Volatile p-cymene inhibited mycelial growth of pathogen at 0.50 μL/ml, whereas γ-terpinene had an inhibitory effect at 0.05, 0.10, and 0.50 μL/ml. Transcript level of γ-terpinene gene increased after 24 h of treatment. As a conclusion, heat treatment enhanced antifungal activity by volatile terpenoids synthesized by thermo-dependent synthesis pathway of them in thyme. |
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| Keywords: | Essential oil Heat treatment Gas chromatography-mass spectrometry Monoterpene |
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