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In Vitro Production of Equine Embryos and Cloning: Today's Status
Affiliation:1. In Vitro Clonagem Animal S/A, Mogi Mirim, Brazil;2. Crestview Genetics, Buenos Aires, Argentina;1. Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan;2. Centre for Artificial Insemination and Embryo Transfer, University for Veterinary Sciences Vienna, Vienna, Austria;3. Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan;4. Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan;1. Universidad de Buenos Aires, Instituto de Investigación y Tecnología en Reproducción Animal, Facultad de Ciencias Veterinarias, Cátedra de Teriogenología, Ciudad Autónoma de Buenos Aires, Argentina;2. BET Labs/Biorelease Technologies LLC, Lexington, KY;1. Department of Large Animal Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA;2. Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA;3. Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA;4. Equine Reproduction Center, Peterson & Smith Equine Hospital, Ocala, Florida, USA
Abstract:Equine reproductive technologies, such as cloning and intracytoplasmic sperm injection (ICSI) have become routine laboratory practice in many parts of the world. In this review, we will discuss novel cloning approaches that have just recently become commercial, update the latest cloning results at Crestview Genetics in Argentina and at In Vitro Clonagem in Brazil, and discuss differences in techniques and approach within the American equine ICSI market from four different laboratories commercially producing equine ICSI.
Keywords:Animal cloning  Somatic cell nuclear transfer  Intracytoplasmic sperm injection  Commercial applications
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