Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing |
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Institution: | 1. Department of Archaeological Conservation, Faculty of Archaeology, Cairo University, Giza, Egypt;2. Department of Construction Engineering, Technical University of Catalonia, Barcelona, Spain;3. Department of Geotechnical Engineering and Geo-Sciences, Technical University of Catalonia, Barcelona, Spain |
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Abstract: | Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication. |
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Keywords: | Hairpin-RNA Transgenic sweet orange Hypersusceptibility |
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