| 外来入侵植物假臭草总黄酮提取工艺研究 |
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| 引用本文： | 汤丽昌,王宁,李慧,杨先会,邓世明. 外来入侵植物假臭草总黄酮提取工艺研究[J]. 安徽农业科学, 2011, 39(30): 18594-18595,18598 |
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| 作者姓名： | 汤丽昌 王宁 李慧 杨先会 邓世明 |
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| 作者单位： | 海南大学热带生物资源教育部重点实验室,海南海口,570228 |
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| 基金项目： | 国家自然科学基金项目(30860068) |
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| 摘 要： | [目的]优选假臭草中总黄酮的提取工艺。[方法]在单因素试验的基础上,采用L9（33）正交试验,以4＇,5,6,7-四甲氧基黄酮为对照品,以假臭草中总黄酮含量为指标,研究假臭草中总黄酮的提取工艺。[结果]假臭草中总黄酮的最佳提取工艺为：提取时间2.5 h,甲醇浓度40%（V/V）,料液比为1∶20（g/ml）。在该条件下,假臭草中总黄酮含量为1.36%。[结论]该提取工艺合理、稳定、可行。
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| 关 键 词： | 假臭草 总黄酮 正交试验 提取工艺 紫外可见分光光度法 |
Extraction Technology of Total Flavonoids in the Invasive Plant-Eupatorium catarium Veldkamp |
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| TANG Li-chang et al. Extraction Technology of Total Flavonoids in the Invasive Plant-Eupatorium catarium Veldkamp[J]. Journal of Anhui Agricultural Sciences, 2011, 39(30): 18594-18595,18598 |
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| Authors: | TANG Li-chang et al |
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| Affiliation: | TANG Li-chang et al(Key Laboratory of Tropic Biological Resources,MOE,Hainan University,Haikou,Hainan 570228) |
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| Abstract: | [Objective] The aim was to optimize the technology for extracting total flavonoids from Eupatorium catarium.[Method] Based on the single-factor test,the extraction process of total flavonoids from Eupatorium catarium was optimized by using L9(33) orthogonal test with 4′,5,6,7-tetramethoxyflavone as standard and the total flavonoids content as index.[Result] The optimum conditions of ultrasonic extraction were 2.5 hours as extraction time,40% methonal(V/V) as solvent,solid-liquid ratio 1∶20(g/ml).The content of total flavonoids in Eupatorium catarium was 1.36%.[Conclusion] This extraction process is rational,stable and practicable. |
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| Keywords: | Eupatorium catarium Veldkamp Total flavonoids Orthogonal test Extraction technology Ultraviolet-visible spectrophotometry |
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