| 棉花DNA提取方法综述 |
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| 引用本文： | 谭燕华,彭存智,王冬梅.棉花DNA提取方法综述[J].安徽农业科学,2011,39(32):19684-19685,19688. |
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| 作者姓名： | 谭燕华 彭存智 王冬梅 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海南海口571101;农业部转基因植物及植物用微生物环境安全监督检测中心(海口),海南海口571101 |
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| 基金项目： | 中央级公益性科研院所基本科研费(ITBB110604) |
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| 摘 要： | 棉花是我国重要的经济作物之一,富含棉酚、多糖、单宁等其他干扰物质,因此其DNA的提取和纯化较其他作物更为困难。文中对棉花DNA提取方法进行了讨论,其中SDS法和CTAB法是常用的2种提取方法,而CTAB法及其改良法提取的效果最好。
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| 关 键 词： | 棉花 DNA提取 综述 |
Progress on Extraction Methods of DNA from Cotton |
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| TAN Yan-hua et al.Progress on Extraction Methods of DNA from Cotton[J].Journal of Anhui Agricultural Sciences,2011,39(32):19684-19685,19688. |
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| Authors: | TAN Yan-hua |
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| Institution: | TAN Yan-hua et al(Institue of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101) |
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| Abstract: | Cotton was one of important economic crops in China.Because of the rich gossypol,polysaccharides,tannins and other interfering compounds in cotton,the extraction and purification of DNA were much more difficult than other crops.The extraction methods of cotton's genomic DNA were reviewed.In these extraction methods,SDS method and CTAB method were commonly used,and the CTAB method and its modified method resulted in best extraction outcome. |
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| Keywords: | Cotton Extraction of DNA Summary |
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