| 银杏叶中总黄酮的提取工艺和含量测定的研究 |
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| 引用本文： | 董宏博,崔桂花,赵文秀. 银杏叶中总黄酮的提取工艺和含量测定的研究[J]. 安徽农业科学, 2011, 39(30): 18518-18520 |
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| 作者姓名： | 董宏博 崔桂花 赵文秀 |
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| 作者单位： | 吉林医药学院,吉林吉林,132013 |
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| 摘 要： | [目的]研究银杏叶黄酮类成分的提取和含量测定方法。[方法]对银杏叶中总黄酮的乙醇提取法和超声提取法进行研究并加以改进,通过单因素试验确定银杏叶总黄酮的最佳提取工艺条件。以显色法测定提取液中总黄酮的含量,并对显色测定法进行改进。[结果]超声提取法提出率为0.83%,索氏提取法提出率为1.10%,超声提取与索氏提取结合法提出率为1.37%。最佳提取条件为：超声时间20 min、乙醇浓度60%、料液比1∶50（g/ml）、85℃下加热回流提取2 h。[结论]优选出银杏叶中总黄酮的最佳提取工艺,为进一步研究和应用提供依据。
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| 关 键 词： | 银杏叶（GINKGO FOLIUM） 总黄酮 含量测定 |
Extraction Process and Content Determination of Total Flavonoids from GINKGO FOLIUM |
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| DONG Hong-bo et al J. Extraction Process and Content Determination of Total Flavonoids from GINKGO FOLIUM[J]. Journal of Anhui Agricultural Sciences, 2011, 39(30): 18518-18520 |
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| Authors: | DONG Hong-bo et al J |
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| Affiliation: | DONG Hong-bo et al J(ilin Medical College,Jilin,Jilin 132013) |
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| Abstract: | [Objective] The aim was to study on the extraction and content deter mination methods for total flavonoids from GINKGO FOLIUM.[Method] Both the ultrasonic extraction and Soxhlet extraction methods were studied and improved.Content of total flavonoids in extraction solution was deter mined by coloration method.The optimal extraction condition for the extraction of total flavonoids from GINKGO FOLIUM was deter mined by the single-factor test.[Result] The extraction rates of ultrasonic extraction and Soxhlet e... |
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| Keywords: | GINKGO FOLIUM Total flavonoids Content deter mination |
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