| 添加剂对纤维素酶耐温性的影响 |
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| 引用本文： | 姚秀清,孙丽萍,周丹丹.添加剂对纤维素酶耐温性的影响[J].安徽农业科学,2011,39(30):18395-18396,18410. |
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| 作者姓名： | 姚秀清 孙丽萍 周丹丹 |
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| 作者单位： | 辽宁石油化工大学环境与生物工程学院,辽宁抚顺,113001 |
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| 摘 要： | 为提高纤维素酶在较高温度条件下的耐温性,考察5种添加剂（聚乙二醇、甘油、木糖醇、氯化钙、黄原胶）对纤维素酶酶活的影响。结果表明,在58℃、保存24 h条件下,聚乙二醇、甘油、木糖醇、黄原胶对纤维素酶酶活有保护效应,较未添加时的相对酶活有所增加。木糖醇的保护效应最为显著,相对酶活由0.59提高到0.94;而氯化钙对酶活有抑制效应,相对酶活由0.59降低为0.49。
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| 关 键 词： | 纤维素酶 耐温性 添加剂 木糖醇 |
Effects of Additives on the Thermostability of Cellulase |
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| YAO Xiu-qing et al.Effects of Additives on the Thermostability of Cellulase[J].Journal of Anhui Agricultural Sciences,2011,39(30):18395-18396,18410. |
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| Authors: | YAO Xiu-qing |
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| Institution: | YAO Xiu-qing et al(School of Environment and Bioengineering,Liaoning Shihua University,Fushun,Liaoning 113001) |
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| Abstract: | In order to improve the thermostability of cellulase under higher temperature,the effects of five additives including polyethylene glycol(PEG),glycerol,xylitol,calcium chloride and xanthan gum on cellulose activities were studied.The results showed that protective effect was found in the presence of polyethylene glycol,glycerol,xylitol and xanthan gum under the condition of 58 ℃ and preserved 24 h.The cellulase activities with the addition of additives were higher than that without additives.Among the four additives,protective effect of xylitol on the cellulase activities was most evident.Relative cellulase activity was increased from 0.59 to 0.94.While inhibiting effect of calcium chloride on the cellulase activities was existed with relative cellulase activity decreased from 0.59 to 0.49. |
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| Keywords: | Cellulase Thermostability Additive Xylitol |
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