| 濒危植物大花杓兰种子离体培养条件初探 |
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| 引用本文： | 朴仁哲,王艳丽,赵洪颜.濒危植物大花杓兰种子离体培养条件初探[J].安徽农业科学,2011,39(30):18428-18429,18445. |
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| 作者姓名： | 朴仁哲 王艳丽 赵洪颜 |
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| 作者单位： | 1. 延边大学农学院,吉林延吉,133002
2. 汪清县农业局,吉林汪清,133200 |
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| 基金项目： | 延边大学州校合作项目(延大科合字[2005]第02号) |
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| 摘 要： | 目的]该研究旨在探讨野生大花杓兰种子人工大量繁殖的培养条件。方法]以大花杓兰半成熟种子为试验材料,对其在无菌条件下的萌发进行了研究。结果]结果表明,0.5%的NaClO预处理种子160 min,pH 5.8及添加0.5 mg/L 6-BA和马铃薯浸提物的Harvais培养基是大花杓兰种子无菌萌发的最佳培养条件。结论]该研究为大花杓兰种子的组培繁殖提供依据。
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| 关 键 词： | 大花杓兰 种子 离体培养 |
Preliminary Exploration on In Vitro Seed Culture Conditions of Cypripedium macranthos Sw |
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| PIAO Ren-zhe et al.Preliminary Exploration on In Vitro Seed Culture Conditions of Cypripedium macranthos Sw[J].Journal of Anhui Agricultural Sciences,2011,39(30):18428-18429,18445. |
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| Authors: | PIAO Ren-zhe |
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| Institution: | PIAO Ren-zhe et al(College of Agriculture,Yanbian University,Yanji Jinlin 133002) |
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| Abstract: | Objective] This study aimed to investigate the culture conditions for artificial mass propagation of wild Cypripedium macranthos seeds.Method] Semi-mature seeds of Cypripedium macranthos were used as test materials for study the germination under sterile conditions.Result] The results showed that the optimal culture conditions for sterile germination of Cypripedium macranthos seeds were pretreatment with 0.5% of NaClO for 160 min,pH 5.8 and using Harvais medium containing 0.5 mg/L of 6-BA and potato extr... |
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| Keywords: | Cypripedium macranthos Sw Seeds In vitro culture |
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