不同类型肠梗阻血清中丙二醛的测定和细菌移位的实验研究

目的：比较在不同类型肠梗阻中和不同范围绞窄性肠梗阻中丙二醛的差异和细菌移位情况并观察ＭＤＡ和细菌移位的相互关系。方法：健康１月龄新西兰小兔４０只，随机分成三组并制成不同的模型；（１）绞窄性肠梗阻组。（２）单纯性肠梗阻组；（３）对照组。测定各种肠梗阻前和梗阻１２ｈ后血清中ＭＤＡ的含量，并行血细菌培养。结果 ：Ａ组ＭＤＡ明显高于Ｂ组和Ｃ组；而Ｂ组和Ｃ组无差异；绞窄肠袢范围大者ＭＤＡ高于范围小者。

An experimental study of serum malondialdehyde content and bacterial translocation in various kinds of bowel obstruction

Objective:To compare serum malondialdehyde (MDA) content and bacterial translocation in various kinds of bowel obstruction and in those with different lengths of intestinal loop strangulated.The relation between serum MDA content and the incidence of bacterial translocation was also studied.Methods:40 healthy New Zealand rabbits were divided into 3 groups and made to be different kinds of mode:Group A with strangulated bowel obstruction;Group B with simple obstruction and Group C as control.Serum MDA content were measured 12 hours after obstruction and blood cultures also taken.Results: Serum MDA contents in Group A were markedly higher than those in Group B and Group C( P< 0.05 and P <0.01),whilst there was no difference between Group B and Group C.Regarding to Group A itself,there were higher MDA contents in Group A 100 with the long strangulated bowel loop than those in Group A 40 with the short one ( P <0.05).The incidence of bacterial translocation in which no difference was seen between Group B and Group C,was higher in Group A + Group B than in Group C( P <0.05),was higher in Group A than in Group B( P < 0.01 ).Between the length of bowel strangulated and this incidence no correlation could be found.Conclusious:In clinical point of view it is of significance to monitor free radical and to watch for bacterial translocation for establishing a precise diagnosis of bowel obstraction in detail,evaluating the relative length of bowel loop strangulated,deciding what kind of and when the surgical procedure to perform and at last for the prognosis forecasting.