| 抗病南瓜种质482-2离体快繁技术研究 |
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| 引用本文： | 蔡祖国,周会萍,赵一鹏,杨鹏鸣,周俊国.抗病南瓜种质482-2离体快繁技术研究[J].北方园艺,2009(1). |
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| 作者姓名： | 蔡祖国 周会萍 赵一鹏 杨鹏鸣 周俊国 |
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| 作者单位： | 河南科技学院园林学院,河南新乡,453003;新乡学院生命科学与技术系,河南新乡,453003 |
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| 基金项目： | 河南科技学院重点科技攻关资助项目 |
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| 摘 要： | 以抗病南瓜种质482-2的种子为起始材料,通过对组培条件下的培养基筛选试验,建立离体快繁技术体系.结果表明:MS+6-BA 1.0 mg/L是南瓜子叶诱导不定芽的适宜培养基,不定芽在MS+6-BA 1.0 mg/L培养基中增殖系数能够达到4.0,在MS+IBA 0.2 mg/L培养基上不定芽生根率达到83.3%.再生小植株经驯化、移栽,成活率可达95%.
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| 关 键 词： | 南瓜 组织培养 离体再生 离体快繁 |
Studies on the Rapid Propagation in Vitro of Disease Resistant Pumpkin Germplasm 482-2 |
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| CAI Zu-guo,ZHOU Hui-ping,ZHAO Yi-peng,YANG Peng-ming,ZHOU Jun-guo.Studies on the Rapid Propagation in Vitro of Disease Resistant Pumpkin Germplasm 482-2[J].Northern Horticulture,2009(1). |
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| Authors: | CAI Zu-guo ZHOU Hui-ping ZHAO Yi-peng YANG Peng-ming ZHOU Jun-guo |
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| Institution: | 1.School of Landscape and Architecture;Henan Institute of Science and Technology;Xinxiang;Henan 453003;China;2.College of Economic and Management;Xinxiang Institute;China |
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| Abstract: | Took the seeds of disease resistant pumpkin germplasm 482-2 as the initial material,rapid propagation in vitro was performed by medium selecting.The result showed that the suitable medium for pumpkin adventitious buds regeneration from cotyledon was MS medium supplemented with BA 1.0 mg/L,on the medium of MS supplemented with BA 1.0 mg/L,the multiplication rate was 4.0,and on the rooting medium of MS supplemented with IBA 0.2 mg/L,radication rate reached 83.3%.After acclimatization and transplantation,the r... |
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| Keywords: | Pumpkin Tissue culture Regeneration in vitro Rapid propagation in vitro |
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