体外受精不同时间等因素对体外受精效果的影响

本试验对牛体外受精不同时间、不同的培养液成分和培养方法等对奶牛体外受精后的卵裂率、囊胚发育率的影响进行了研究。试验包括:(1)牛体外受精不同时间(8h、20h)对奶牛体外受精后的卵裂率、囊胚发育率的影响;(2)不同的培养液成分对奶牛体外受精早期胚胎发育的影响。研究结果表明:(1)牛体外受精时间20h对奶牛体外受精后的卵裂率(78%)好于体外受精8h组(76%),但囊胚发育率前者不如后者好(20.51%VS23.68%),两者间差异不显著(P0.05)。(2)作为早期胚胎的培养液IVD101、TCM199培养系的卵裂率分别为76%、74%,而囊胚率却分别为22.37%、22.97%,TCM199培养系好于IVD-101,但两者间差异不显著(P0.05)。

Influence of Different Time of IVF and Other Factors on Effect of IVF

This experiment researched cleavage rate and blastocyst rate of IVF with different IVF time（8h, 20h）, medium components, cluture methods, and also researched the effect of different culture medium composition on early embryonic development. The result showed as follows. （1）20h group the cleavage rate of in vitro fertilization（78%） was better than in vitro fertilization 8 h group（76%）, However, the former was not better than the latter（20.51% VS 23.68%） in blastocyst rates, but there were no significant difference between them（P〉0.05）. （2）the cleavage rate were 76% and 74% respectively by early embryo cuhrue medium IVD101 and TCM199, while the blastocyst rate were spectively 22.37% and 22.97%, TCM199 culture system was better than IVD-101. However,there were no significant difference between them（P〉0.05）.