奥利亚罗非鱼和尼罗罗非鱼几种谷胱甘肽S-转移酶基因克隆与分析

通过RT-PCR法和RACE法,分别从奥利亚罗非鱼(Oreochromisco aureus)肝脏获得alpha、rho1、rho2型GST(GSTA、GSTR1、GSTR2)基因cDNA全序列,从尼罗罗非鱼(Oreochromis nilotica)肝脏获得GSTR1、GSTR2基因cDNA全序列。结果表明:奥利亚罗非鱼GSTA基因cDNA全序列为933bp,5′非翻译区(5′-UTR)为98bp,3′非翻译区(3′-UTR)为166bp,开放阅读框(ORF)为669bp,编码222个氨基酸。奥利亚、尼罗罗非鱼GSTR1基因ORF均为681bp、均编码226个氨基酸;奥利亚、尼罗罗非鱼GSTR2基因ORF均为693bp,均编码230个氨基酸。氨基酸序列同源性比较和系统进化分析均表明,罗非鱼GST基因与鱼类GST同源性较高,与哺乳类、鸟类、两栖类GST同源性较低。

Molecular cloning and sequence analysis of alpha- and rho- classes of glutathione S-transferases in two tilapias,Oreochromisco aureus and Oreochromis nilotica

The full-length cDNAs encoding alpha-class,rho1-class,rho2-class GST(GSTA,GSTR1,and GSTR2) were cloned and sequenced from the blue tilapia(Oreochromisco aureus),and the GSTR1,GSTR2 were cloned and sequenced from the Nile tilapia(Oreochromis nilotica) by RT-PCR and RACE method.The full-length blue tilapia GSTA cDNA was 933 bp in length,containing an open reading frame of 669 bp(encoding 222 amino acids),flanked by 98 bp 5′UTR and 166 bp 3′UTR.Both the blue tilapia and Nile tilapia GSTR1 cDNA ORF were 681 bp(encoding 226 amino acids),and both the blue tilapia and Nile tilapia GSTR2 cDNA ORF were 693 bp(encoding 230 amino acids),respectively.Homology and the phylogenetic tree constructed based on GST sequences showed that the tilapia GSTs amino acid sequence were highly conserved with fish,and had low homologous relationship with amphibian,bird and mammal GSTs.