| 菲降解细菌L2的培养条件研究及菲降解率测定 |
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| 引用本文： | 祝儒刚,李玉双,钟鸣,周启星,刘少霞. 菲降解细菌L2的培养条件研究及菲降解率测定[J]. 安徽农业科学, 2006, 34(3): 407-408 |
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| 作者姓名： | 祝儒刚 李玉双 钟鸣 周启星 刘少霞 |
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| 作者单位： | 1. 沈阳农业大学,辽宁省农业生物技术重点实验室,辽宁,沈阳,110161
2. 中国科学院沈阳应用生态研究所,中国科学院陆地生态过程重点实验室,辽宁,沈阳,110016 |
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| 基金项目： | 国家863项目(2004AA649060)，中国科学院陆地生态过程重点实验室开放课题 |
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| 摘 要： | 通过选择性富集培养,从沈抚灌区石油污染土壤中分离到1株菲降解细菌L2,该菌株能以菲为唯一碳源和能源生长。通过对L2菌株培养条件优化,确定其最佳培养条件为pH值7.0~7.2,温度28~30℃,150 ml容积三角瓶装液量50 ml。并测定了L2菌株对不同浓度菲的降解率,结果表明,在培养7 d后L2菌株对培养基中浓度为501、001、50 mg/ml的菲的降解率分别为100%、96.4%和97.4%。
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| 关 键 词： | 菲 降解率 培养条件 |
| 文章编号： | 0517-6611（2006）03-0407-02 |
| 收稿时间： | 2005-09-29 |
| 修稿时间： | 2005-09-29 |
Degradation Rate of Phenanthrene-degrading Strain L2 and its Cultural Condition |
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| ZHU Ru-gang et al. Degradation Rate of Phenanthrene-degrading Strain L2 and its Cultural Condition[J]. Journal of Anhui Agricultural Sciences, 2006, 34(3): 407-408 |
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| Authors: | ZHU Ru-gang et al |
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| Affiliation: | Shenyang Agricultural University, The Key Laboratory of Agricultural Biotechnology of Liaonlng Province, Shenyang, Liaoning 110161 |
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| Abstract: | A phenanthrene-degrading strain L2 was isolated from oil-contaminated soil in Shenfu irrigation area by selective enrichment culture.The strain was able to grow on phenanthrene as a sole carbon and energy source for growth.It was suggested that the optimization of culture condition was: pH 7.0～(7.2),28～30 ℃,50 ml inventory/150 ml flask.Meanwhile,the degradation rate of phenanthrene under different phenanthrene concentrations was determined.It was shown that after 7 days of cultivation,the degradation rate under phenanthrene concentrations of 50 mg/ml,100 mg/ml,150 mg/ml were 100%,96.4% and 97.4% respectively. |
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| Keywords: | Phenanthrene Culture condition Biodegradation |
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