| 龙眼转醛醇酶基因的克隆与原核表达 |
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| 引用本文： | 游向荣,许鸿川,梁文裕,郑少泉,陈伟.龙眼转醛醇酶基因的克隆与原核表达[J].北方园艺,2009(12). |
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| 作者姓名： | 游向荣 许鸿川 梁文裕 郑少泉 陈伟 |
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| 作者单位： | 1. 福建农林大学,生命科学学院,福建,福州,350002
2. 福建省农业科学院,果树研究所,福建,福州,350013 |
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| 基金项目： | 国家自然科学基金资助项目，福建省自然科学基金资助项目，教育部博士点基金资助 |
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| 摘 要： | 转醛醇酶作为磷酸戊糖途径非氧化阶段的关键酶,在调节植物PPP对环境胁迫的应答中起重要作用.该研究应用RACE方法克隆了龙眼(Dimocarpus longan Lour.)花芽中转醛醇酶的基因,获得一段长度为1 655 bp的cDNA,其中包括一个1 320 bp的开放阅读框,已登陆GenBank,登陆号为FJ472991(GI:217795375).将转醛醇酶全长cDNA在大肠杆菌中表达,获得一个约55 kD带有组氨酸标签的外源蛋白.RT-PCR结果显示,TAL mRNA在龙眼成花逆转花芽中有较明显的表达量上调,说明TAL在一定程度上影响了龙眼正常成花.
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| 关 键 词： | 龙眼 转醛醇酶 基因 克隆 原核表达 |
Cloning and Prokaryotic Expression of Transaldolase from Longan |
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| YOU Xian-rong,XU Hong-chuan,LIANG Wen-yu,ZHENG Shao-quan,CHEN Wei.Cloning and Prokaryotic Expression of Transaldolase from Longan[J].Northern Horticulture,2009(12). |
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| Authors: | YOU Xian-rong XU Hong-chuan LIANG Wen-yu ZHENG Shao-quan CHEN Wei |
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| Institution: | YOU Xiang-rong1,XU Hong-chuan1,LIANG Wen-yu1,ZHENG Shao-quan2,CHEN Wei1(1.College of Life Science,Fujian Agriculture , Forestry University,Fuzhou,Fujian 350002,China,2.Fruit Research Institute,Fujian Academy of Agriculture Science,Fujian 350013,China) |
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| Abstract: | The pentose-phosphate pathway(PPP)plays a pivotal role in carbohydrate metabolism,as a key enzyme of the nonoxidative PPP branch,transaldolase showed advertent response to environmental stress regulation.We have isolated a cDNA encoding transaldolase,an enzyme of the pentose-phosphate pathway,from longan(Dimocarpus longan Lour.).RACE method was used and a full length of 1 655 bp cDNA,with a 1 320 bp open reading frame,was obtained(GenBank access number:FJ479618).Transaldolase cDNA was expressed in E.coli,an... |
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| Keywords: | Longan Transaldolase Gene Clone Prokaryotic expression |
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