柑橘衰退病毒研究进展

综合有关文献,就柑橘衰退病毒(Citrustristezavirus,CTV)的生物学特征、分子生物学、抗病毒基因工程和柑橘衰退病的检测、鉴定及防治作一综述。重点讨论CTV株系分化、基因组和亚基因组RNA、CTV的血清学和分子生物学检测方法。目前的研究表明,CTV通常以不同株系的混合物存在,并且和其他病原形成复合侵染,各分离物之间存在明显的遗传多样性。不同株系的分离纯化、各株系间的交叉保护及CTV和橘蚜的互作关系还有待深入的研究。

Advances in Research of Citrus tristeza virus

In this paper the biological characterization and molecular biology of Citrus tristeza virus(CTV),gene engineering for CTV-resistance of citrus,the detection、identification and control of CTV were presented and the great attention is particu-larly focused on CTV strains differentiation,genomic and subgenomic RNA of CTV,the serological and molecular biological detection techniques.The studies showed that CTV usually exists as a complex of isolates or strains they may differ greatly in pathogenicity and biological characterization.Genetic diversity among CTV isolates or strains was also revealed.Further re-search is needed to focus on the separation and purification of CTV isolates,reliable and simple methods for the CTV isolates detection and differentiation,approaches for the mild strain cross-protection and the interaction between CTV and brown cit-rus aphid(Toxoptera citricida)