首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Cellular Immune Responses to 35 kDa Recombinant Antigen of Mycobacterium avium paratuberculosis
Authors:S H Basagoudanavar  P P Goswami  V Tiwari
Institution:(1) National Biotechnology Centre, Indian Veterinary Research Institute (IVRI), Izatnagar, 243 122, India;(2) Present address: Pennsylvania State University College of Medicine, Hershey, PA, USA
Abstract:Mycobacterium avium paratuberculosis is the causative agent of Johne disease, a chronic ulcerative intestinal condition in ruminant animals. Owing to the predominance of cellular response in subclinical forms of the infection, identification of M. a. paratuberculosis antigens eliciting host cell-mediated immune (CMI) reaction is crucial for early control of the disease. A 35 kDa protein of M. a. paratuberculosis was studied for its ability to elicit CMI responses using a mouse model. Lymphoproliferation and IFN-γ response were used to measure the CMI response. Recombinant 35 kDa protein (P35) stimulated proliferation of mouse mononuclear splenocytes sensitized with M. a. paratuberculosis. The P35 elicited increased nitrite production from mononuclear splenocytes from M. a. paratuberculosis-sensitized mice. In addition, RT-PCR-based semiquantitative IFN-γ measurement showed that stimulation with P35 is associated with significant expression of IFN-γ mRNA in M. a. paratuberculosis-sensitized mouse splenocytes. The results indicate that the 35 kDa protein of M. a. paratuberculosis is associated with CMI response in the host.
Keywords:cellular immune response  35 kDa protein  Mycobacterium avium paratuberculosis
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号