Cellular Immune Responses to 35 kDa Recombinant Antigen of Mycobacterium avium paratuberculosis |
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Authors: | S H Basagoudanavar P P Goswami V Tiwari |
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Institution: | (1) National Biotechnology Centre, Indian Veterinary Research Institute (IVRI), Izatnagar, 243 122, India;(2) Present address: Pennsylvania State University College of Medicine, Hershey, PA, USA |
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Abstract: | Mycobacterium avium paratuberculosis is the causative agent of Johne disease, a chronic ulcerative intestinal condition in ruminant animals. Owing to the predominance
of cellular response in subclinical forms of the infection, identification of M. a. paratuberculosis antigens eliciting host cell-mediated immune (CMI) reaction is crucial for early control of the disease. A 35 kDa protein
of M. a. paratuberculosis was studied for its ability to elicit CMI responses using a mouse model. Lymphoproliferation and IFN-γ response were used
to measure the CMI response. Recombinant 35 kDa protein (P35) stimulated proliferation of mouse mononuclear splenocytes sensitized
with M. a. paratuberculosis. The P35 elicited increased nitrite production from mononuclear splenocytes from M. a. paratuberculosis-sensitized
mice. In addition, RT-PCR-based semiquantitative IFN-γ measurement showed that stimulation with P35 is associated with significant
expression of IFN-γ mRNA in M. a. paratuberculosis-sensitized mouse splenocytes. The results indicate that the 35 kDa protein
of M. a. paratuberculosis is associated with CMI response in the host. |
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Keywords: | cellular immune response 35 kDa protein Mycobacterium avium paratuberculosis |
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