| 猪瘟病毒可视化RT-LAMP检测方法的建立 |
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| 引用本文： | 赵明秋,王伟芳,王波,康艳梅,高健潜,陈金顶.猪瘟病毒可视化RT-LAMP检测方法的建立[J].广东畜牧兽医科技,2011,36(2):26-28,32. |
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| 作者姓名： | 赵明秋 王伟芳 王波 康艳梅 高健潜 陈金顶 |
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| 作者单位： | 华南农业大学兽医学院; |
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| 基金项目： | 国家自然科学基金项目(30771611、31072137); 教育部“长江学者和创新团队发展计划”创新团队项目(TRT0723); 广东省自然科学基金创新团队项目(5200638) |
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| 摘 要： | 通过对GenBank中注册的CSFV序列进行比对分析,针对CSFV NPro/C基因中的保守区域,设计合成了一套RT-LAMP引物,应用含有Npro/C基因的阳性质粒株对RT-LAMP引物进行验证.在成功扩增出特异片段的基础上,用CSFV RNA优化RT-LAMP反应体系和条件,建立了CSFV的可视化RT-LAMP快速...
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| 关 键 词： | 猪瘟病毒 RT-LAMP 快速诊断 |
Establishment of RT-LAMP method for detection of CSFV |
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| Zhao Mingqiu,Wang Weifang,Wang Bo,Kang Yanmei,Gao Jianqian,Chen Jinding.Establishment of RT-LAMP method for detection of CSFV[J].Guangdong Journal of Animal and Veterinary Science,2011,36(2):26-28,32. |
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| Authors: | Zhao Mingqiu Wang Weifang Wang Bo Kang Yanmei Gao Jianqian Chen Jinding |
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| Institution: | Zhao Mingqiu,Wang Weifang,Wang Bo,Kang Yanmei,Gao Jianqian,Chen Jinding*(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | A suit of primers were designed according to a part of Npro/C genes of CSFV from GenBank.The specific fragments were amplified from positive plasmid pMD-CSFV using LAMP method.The RT-LAMP reaction system and condition were optimized with CSFV RNA,so a visualized RT-LAMP method was established.The results showed that CSFV RNA could be detected specifically and the RNA of PRRSV,FMDV and JEV all were detected negative.As low as 0.1pg/μL CSFV RNA could be detected. |
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| Keywords: | Classical swine fever virus RT-LAMP rapid detection |
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