| 超数排卵对家兔早期胚胎体外发育的影响 |
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| 引用本文： | 曹君君.超数排卵对家兔早期胚胎体外发育的影响[J].广东畜牧兽医科技,2011,36(2):18-20. |
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| 作者姓名： | 曹君君 |
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| 作者单位： | 汕头大学医学院实验动物中心; |
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| 摘 要： | 为探讨超数排卵对家兔早期胚胎体外发育能力的影响,收集自然发情组和超数排卵处理组交配72 h后家兔胚胎,对经筛选合格的胚胎以小鼠成纤维细胞为饲养层进行体外培养,以自然发情交配的家兔胚胎为对照,观察培养96 h贴壁情况和内细胞团传代能力.超数排卵处理的家兔交配后经筛选所获得的可用胚胎数量增多;其96 h贴壁率和成功传代内细...
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| 关 键 词： | 家兔 胚胎 体外培养 传代 |
Influence of superovulation on growth of early rabbit embryos in vitro |
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| Cao Jun-Jun.Influence of superovulation on growth of early rabbit embryos in vitro[J].Guangdong Journal of Animal and Veterinary Science,2011,36(2):18-20. |
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| Authors: | Cao Jun-Jun |
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| Institution: | Cao Jun-Jun(Shan Tou University Medicine College Laboratory Animal CenterGuang Dong,Shan Tou 515041) |
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| Abstract: | To explore the effect of superovulation on developmental potential of early rabbit embryos,72 hour-aged rabbit embryos were collected from natural intercourse rabbits and superovulation-treated rabbit,respectively.The embryos were selected according quality condition and subsequently cultivated on mouse fibroblast cell layers in vitro.The attachment of embryos in 96h culture was observed and the viability of Inner Cell Mass(ICM) was evaluated in passage culture.The results indicated that superovulation did ... |
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| Keywords: | Rabbit Embryos Cultivation in vitro Passage culture |
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